Project description:Interventions: Gold Standard:tissue or cytopathology;Index test:secreted LncRNA
Primary outcome(s): Differential expression of LncRNA in peripheral blood exosomes of patients
Study Design: Single arm
Project description:To explore the mechanisms underlying the radioresistance of hypopharyngeal carcinoma, we first established specifically radioresistant FaDu cell line (FaDu-RR cells) derived from FaDu cell lines by repeatedly exposing to different doses of ionizing radiation. Then, the aberrantly expressed mRNAs and IncRNAs were detected using microarrays and their bioinformatics were analyzed.
Project description:Nu61, a radiation-resistant human tumor xenograft, was selected from a parental radiosensitive tumor SCC-61 by eight serial cycles of passage in athymic nude mice and in vivo irradiation. Obtained tumors were profiled using Affymetrix U133A arrays. Most abundant gene pattern associated with radioresistant phenotype was presented by IFN-inducible, Stat1-dependent pathway Keywords: Pair-wise comparison of radiosensitive vs radioresistant tumors; time course of irradation response
