Project description:In the absence of light, the heterotrophic microalga Crypthecodinium cohnii accumulates lipids rich in docosahexaenoic acid (DHA). However, limited genetic data restricts understanding of its metabolism, especially DHA synthesis with ethanol. Next-generation sequencing identified numerous transcripts for fatty acid and carbohydrate production. qRT-PCR confirmed increased expression of genes linked to energy, carbohydrate, lipid, terpenoid, and polyketide metabolism. Upregulation of genes in ethanol metabolism, fatty acid synthesis, glycerophospholipid, and pyruvate pathways raised acetyl-CoA and key metabolite precursors.

2026-01-20 | GSE316578 | GEO

Crypthecodinium cohnii

Project description:Transcriptome profiling of UV-C irradiated Crypthecodinium cohnii

| PRJNA853487 | ENA

Rewiring the metabolic network to increase docosahexaenoic acid productivity in Crypthecodinium cohnii by fermentation supernatant-based adaptive laboratory evolution

Project description:To overcome the inhibition caused by the fermentation supernatant in the late fermentation stage of docosahexaenoic acid (DHA)-producing Crypthecodinium cohnii, fermentation supernatant-based adaptive laboratory evolution (FS-ALE) was conducted. The cell growth and DHA productivity of the evolved strain (FS280) obtained after 280 adaptive cycles corresponding to 840 days of evolution were increased by 161.87% and 311.23%, respectively, at 72 h under stress conditions and increased by 19.87% and 51.79% without any stress compared with the starting strain, demonstrating the effectiveness of FS-ALE.

2021-11-27 | PXD030028 |

Staphylococcus cohnii

Project description:Staphylococcus cohnii Raw sequence reads

| PRJNA290930 | ENA

Crypthecodinium

Project description:Crypthecodinium sp. overview

| PRJNA1058075 | ENA

Improved lipid production and transcriptomic profiling in Crypthecodinium cohnii with alternative ethanol treatment

Project description:Improved lipid production and transcriptomic profiling in Crypthecodinium cohnii with alternative ethanol treatment

| PRJNA1403410 | ENA

Crypthecodinium sp.

Project description:Temperature-Crypthecodinium sp. SUN-RNA-seq