Rewiring the metabolic network to increase docosahexaenoic acid productivity in Crypthecodinium cohnii by fermentation supernatant-based adaptive laboratory evolution

ABSTRACT: To overcome the inhibition caused by the fermentation supernatant in the late fermentation stage of docosahexaenoic acid (DHA)-producing Crypthecodinium cohnii, fermentation supernatant-based adaptive laboratory evolution (FS-ALE) was conducted. The cell growth and DHA productivity of the evolved strain (FS280) obtained after 280 adaptive cycles corresponding to 840 days of evolution were increased by 161.87% and 311.23%, respectively, at 72 h under stress conditions and increased by 19.87% and 51.79% without any stress compared with the starting strain, demonstrating the effectiveness of FS-ALE.

ORGANISM(S): Crypthecodinium Cohnii

SUBMITTER: Weiwen Zhang  

PROVIDER: PXD030028 | iProX | Sat Nov 27 00:00:00 GMT 2021

REPOSITORIES: iProX