Project description:To explore the role of macrophage NFATc3 on steatohepatitis, macrophage-specific Nfatc3 knockin mice (Nfatc3-MKI) were generated. Lyz2-Cre mice were used as control. Mouse bone marrow-derived macrophages (BMDMs) were isolated from Lyz2-Cre and Nfatc3-MKI mice and then were subjected to ChIP sequencing analysis.
Project description:To investigate the effect of TMEM175 on bone marrow-derived macrophages, bone marrow-derived macrophages from WT or Tmem175flox/flox Lyz2-Cre+/- mice were cultured. The cells were then lysed for RNA-seq.
Project description:Bone marrow-derived macrophages (BMMs) from Nedd8(flox/flox) mice or Nedd8(flox/flox; lyz2-cre) mice were stimulated with or without 100ng/ml LPS for 4 hours. The total RNA was prepared and subjected to microarray.
Project description:Bone marrow-derived macrophages (BMMs) from Uba3(flox/flox) mice or Uba3(flox/flox; lyz2-cre) mice were stimulated with or without 100ng/ml LPS for 4 hours. The total RNA was prepared and subjected to microarray.
Project description:To explore the roles of macrophage NFATc3 and ASK1 in metabolic dysfunction-associated steatohepatitis, we generated macrophage-specific Nfatc3 knockout mice (Nfatc3-MKO), macrophage-specific Ask1 knockout mice (Ask1-MKO),macrophage-specific double knockout (MDKO) mice, macrophage-specific Nfatc3 knockin mice (Nfatc3-MKI), and macrophage-specific Ask1 knockin mice (Ask1-MKI). Lyz2-Cre mice were used as control. Eight-week-old male Lyz2-Cre, Nfatc3-MKO, Ask1-MKO, and MDKO mice were treated with methionine- and choline-deficient (MCD) diet for 4 weeks. Subsequently, mouse liver tissues were isolated and subjected to RNA sequencing analysis. In addition, mouse bone marrow-derived macrophages (BMDMs) were isolated from Lyz2-Cre, Nfatc3-MKI, and Ask1-MKI mice and were also subjected to RNA sequencing analysis.
Project description:Alkbh5/Mettl3loxp/loxp mice were crossed with Lyz2-Cre mice to generate macrophage-specific conditional knockout mice for Alkbh5/Mettl3. Bone marrow-derived macrophages (BMDMs) were isolated from these mice, and MeRIP-seq was performed to investigate the impact of the knockout on RNA N6-Methyladenosine
Project description:Bone marrow-derived macrophages (BMMs) from Itga9-Lyz2-cKO and Itga9-Lyz2-cHet mice were stimulated with Porphyromonas gingivalis (Pg) for 2 hours, then subjected to mRNA sequencing.
Project description:To investigate the function OGT in the regulation of M2 polarization of macrophages, we established IL-4-activated bone marrow-derived macrophages (BMDMs) from mice of wild-type control or Lyz2-Cre/Loxp-mediated knockout of OGT. We then performed gene expression profiling analysis using data obtained from RNA-seq of wild-type (WT) and OGT-knockout (OGT-KO) macrophages at two time points during IL-4 (20ng/μl) sitmulation.
Project description:Bone marrow derived macrophages of Lysz-Cre;Catnbtm2Kem(fl/fl) mouse were compared with bone marrow derived macrophage of Catnbtm2Kem(fl/fl) control mouse Total RNA extracted from bone marrow derived macrophage
