Project description:Acute Oak Decline (AOD) is a decline-disease currently spreading in Britain, threatening oak trees. Here, we analyze and compare the proteomes of inner bark tissue sampled from oak stems of trees symptomatic with AOD and non-symptomatic trees.
Project description:Seedlings grown from seeds from open-pollinated mother trees of genotype UF12 were grown and at two months of age used to analyze response to treatment with the fungal pathogen Colletotrichum theobromicola and the oomycete pathogen Phytophthora palmivora.
Project description:Expression diversity of P. ramorum isolates belonging to the NA1 clonal lineage growing on solid CV8 was examined. We found that phenotypes and transcriptomes change when isolates were passing through oak trees.
Project description:Using data from acorns produced by mature oak trees in the eighth year of elevated CO2 (eCO₂), we present evidence that similar effects occur in long-established forests, with negative consequences for seed quality, that impact herbivore nutrition and health. The analysis of acorns from the near-200-year-old oak trees at the Free Air Carbon dioxide (FACE) facility at the Birmingham Institute for Forest Research (BIFoR) revealed that growth under eCO₂ increased the phytate content but decreased the protein content of acorns. Additionally the analysis of protin profiles showed significant differnves in protein abundances in both typoe of samples.
Project description:<p>Plant-leaf endophytic fungi mutualism is essential for plants to adapt to adverse environments. Though introgression has been extensively studied in many plants, the underlying effects of introgression on fungi-driven adaptability still remain unanswered. Here, we performed landscape-scale sampling of natural populations across its typical distribution range in China to disentangle how introgression effects endophytic fungal community within oak leaves. Our results showed that fungal diversity was decreased while fungal co-occurrence network complexity was increased with increasing introgression index, reflecting a “dual adaptation strategy” to respond to environmental challenges. This pattern was also linked to metacommunity: the lowest diversity occurred in metacommunity that characterized by the lowest mean annual temperature and annual precipitate, whereas the highest fungal network complexity appeared in metacommunity that had the highest mean annual temperature and optimal annual precipitate. Importantly, the differential metabolite intensities (e.g., organoheterocyclic compounds, and organoheterocyclic compounds) and climate factors mediated the leaf endophytic fungi-driven adaptability of oak trees. This work not only advances our understanding of plant-microbe interactions in the context of evolutionary ecology but also provides insight into the importance of fungal community restructuring in facilitating plant adaptation to environmental change.</p>
Project description:Sphaerulina musiva is an economically and ecologically important fungal pathogen that causes Septoria stem canker and leaf spot disease of Populus species. To bridge the gap between genetic markers and structural barriers previously found to be linked to Septoria canker disease resistance in poplar, we used hydrophilic interaction liquid chromatography and tandem mass spectrometry to identify and quantify metabolites involved with signaling and cell wall remodeling. Fluctuations in signaling molecules, organic acids, amino acids, sterols, phenolics, and saccharides in resistant and susceptible P. trichocarpa inoculated with S. musiva were observed. The patterns of 222 metabolites in the resistant host implicate systemic acquired resistance (SAR), cell wall apposition, and lignin deposition as modes of resistance to this hemibiotrophic pathogen. This pattern is consistent with the expected response to the biotrophic phase of S. musiva colonization during the first 24 h postinoculation. The fungal pathogen metabolized key regulatory signals of SAR, other phenolics, and precursors of lignin biosynthesis that were depleted in the susceptible host. This is the first study to characterize metabolites associated with the response to initial colonization by S. musiva between resistant and susceptible hosts.
The work (proposal:https://doi.org/10.46936/10.25585/60000891) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.
Project description:Protein lysine acetylation, a dynamic and reversible posttranslational modification, plays a crucial role in several cellular processes including cell cycle regulation, metabolic pathways, enzymatic activities and protein interactions. Brenneria nigrifluens is the pathogen of shallow bark canker of walnut trees and can cause serious disease on walnut trees. Up to now, it is little known about the roles of lysine acetylation in the plant pathogenic bacteria. In the present study, the lysine acetylome of B. nigrifluens was determined by high-resolution LC-MS/MS analysis. In total, we identified 1,866 lysine acetylation sites distributed in 737 acetylated proteins. Bioinformatics results indicate that acetylated proteins participate in many different biological functions in B. nigrifluens. Four conserved motifs, namely, LKac, Kac*F, I*Kac and L*Kac, were identified in this bacterium. Protein interaction network analysis indicates that all kinds of interactions are modulated by protein lysine acetylation. Overall, 14 acetylated proteins are related to the virulence of B. nigrifluens.