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Saccharomyces

ABSTRACT: Poly Synthetic Strains

PROVIDER: PRJNA376801 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5286952_1.fastq.gz	Fastqsanger.gz
	SRR5286952_2.fastq.gz	Fastqsanger.gz
	SRR5286673_1.fastq.gz	Fastqsanger.gz
	SRR5286673_2.fastq.gz	Fastqsanger.gz
	SRR5286953_1.fastq.gz	Fastqsanger.gz

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Project description:To characterize the ecological interactions among S. cerevisiae strains coming from the same geographical area, we examined the fitness of two natural isolates from San Giovese grapes, alone or in competition, in synthetic wine must (SWM). We performed genome-wide analyses in order to identify the genes involved in yeast competition and cooperation.

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Project description:The aim of this study is to phenotype a collection of 27 S. cerevisiae commercial wine strains growing within temperatures (4-45ºC) in both minimal media (SD) and synthetic must (SM) and, taking into account µmax value, to select two strains with divergent phenotype in their capacity to grow at low temperature. To confirm this differential phenotype, we design a competition between both strains during wine fermentations. As expected, at low temperature fermentation, the strain showing a good performance out-competes to the strain growing badly in cold. Finally we aimed to decipher the molecular basis underlying this divergent phenotype by analyzing the genomic, proteomic and transcriptomic differences between both strains at low temperature (15ºC) and optimum temperature (28ºC).

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Genome-wide maps of nucleosome positions in Saccharomyces strains bearing heterologous DNA on yeast artificial chromosomes

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