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Mus musculus

ABSTRACT: Single cell RNA-seq of 444 epithelial cells from the mammary glands of pubescent and adult mice

PROVIDER: PRJNA377129 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5293530_1.fastq.gz	Fastqsanger.gz
	SRR5293530_2.fastq.gz	Fastqsanger.gz
	SRR5293540_1.fastq.gz	Fastqsanger.gz
	SRR5293540_2.fastq.gz	Fastqsanger.gz
	SRR5293550_1.fastq.gz	Fastqsanger.gz

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Project description:Single cell RNA-seq of 346 epithelial cells from the mammary glands of pre-pubescent, pubescent and adult mice

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Project description:This SuperSeries is composed of the SubSeries listed below. Mammary glands were collected from female mice of various ages: pre puberty (2 weeks), pubescent (5 weeks), adult (10 weeks) and pregnant (12.5 days mid-pregnancy). Freshly sorted epithelial cells were submitted to a Fluidigm C1 System machine for single cell capture and cDNA synthesis. In some cases, cells were further sorted into basal and luminal subsets. Cells were visualized under a microscope to ensure integrity of the captured single cells prior to cDNA preparation. Libraries were sequencing on an Illumina HiSeq 2000 to achieve 100bp paired-end reads or on an Illumina NextSeq to achieve 75bp paired-end reads.

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Project description:Single cell RNA-seq of 312 basal epithelial cells from the mammary glands of pregnant and adult mice

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