Project description:Long non-coding RNAs were associated with the development and progression of glaucoma. Our study aim to identify the potential genes in human trabecular meshwork related to primary open-angle glaucoma (POAG).
Project description:To better understand the molecular changes in the aqueous humor (AH) content with glaucoma, we analyzed the microRNA (miRNA) profiles of AH samples from patients with Primary Open Angle Glaucoma (POAG) and Exfoliation Glaucoma (XFG) compared to non-glaucoma controls.
Project description:MicroRNAs were associated with the development and progression of glaucoma. Our study aims to identify the potential miRNAs and target genes in human trabecular meshwork related to primary open-angle glaucoma (POAG).
Project description:Long noncoding RNAs (lncRNAs) are emerging as important regulators in cellular processes and have been showed to be involved in the occurrence and development of various neurodegenerative diseases including glaucoma. The aim of this study is to reveal disease-related extracellular lncRNAs and message RNAs (mRNAs) in aqueous humor (AH) of individual primary open-angle glaucoma (POAG) patients, to determine the potential biomarkers for POAG diagnosis
Project description:Study investigates the biological role of small RNAs present in aqueous humor in patients with primary open-angle glaucoma, pseudoexfoliative glaucoma, and cataract, with the aim of identifying molecular biomarkers and improving understanding of disease mechanisms. Aqueous humor samples were collected during ophthalmic surgery, followed by RNA extraction from the cell-free supernatant using a commercial kit designed for low-input RNA. Small RNA sequencing libraries were prepared using adapter ligation with Unique Molecular Identifiers, reverse transcription, PCR amplification, and magnetic bead purification, and library quality was assessed using a microfluidics-based system. The pooled libraries were sequenced using paired-end sequencing on an Illumina NextSeq platform, and the resulting FASTQ files were used for downstream bioinformatic and machine learning analyses integrating molecular, clinical, and imaging data.
Project description:This study reports the first characterization of the intracellular proteome of peripheral blood mononuclear cells (PBMC) isolated from subjects diagnosed with primary open angle glaucoma (POAG)by shot-gun proteomics. Glaucoma is a chronic optic neuropathy and among the first causes of irreversible blindness on a global scale. Several recent data have pointed out alterations of immune system processes in glaucoma subjects. Very recently, oxygen consumption rate (OCR) and NAD levels have been proposed as biomarkers of disease severity.
Project description:Primary open angle glaucoma (POAG) presents as an irreversible optic neuropathy with corresponding visual field deficits and no other notable symptoms. The pathophysiology of POAG remains poorly understood. Through proteomic analysis of aqueous humour (AH) from POAG patients, we aim to identify changes in protein composition of these samples compared to control samples. This enables us to better understand pathological changes in POAG from a molecular perspective, and may reveal potential diagnostic molecular biomarkers in POAG. We are also interested in correlating differences in AH protein compositions with demographics and clinical severity in POAG patients.