Project description:Long non-coding RNAs were associated with the development and progression of glaucoma. Our study aim to identify the potential genes in human trabecular meshwork related to primary open-angle glaucoma (POAG).
Project description:To better understand the molecular changes in the aqueous humor (AH) content with glaucoma, we analyzed the microRNA (miRNA) profiles of AH samples from patients with Primary Open Angle Glaucoma (POAG) and Exfoliation Glaucoma (XFG) compared to non-glaucoma controls.
Project description:MicroRNAs were associated with the development and progression of glaucoma. Our study aims to identify the potential miRNAs and target genes in human trabecular meshwork related to primary open-angle glaucoma (POAG).
Project description:Long noncoding RNAs (lncRNAs) are emerging as important regulators in cellular processes and have been showed to be involved in the occurrence and development of various neurodegenerative diseases including glaucoma. The aim of this study is to reveal disease-related extracellular lncRNAs and message RNAs (mRNAs) in aqueous humor (AH) of individual primary open-angle glaucoma (POAG) patients, to determine the potential biomarkers for POAG diagnosis
Project description:Primary open angle glaucoma (POAG) presents as an irreversible optic neuropathy with corresponding visual field deficits and no other notable symptoms. The pathophysiology of POAG remains poorly understood. Through proteomic analysis of aqueous humour (AH) from POAG patients, we aim to identify changes in protein composition of these samples compared to control samples. This enables us to better understand pathological changes in POAG from a molecular perspective, and may reveal potential diagnostic molecular biomarkers in POAG. We are also interested in correlating differences in AH protein compositions with demographics and clinical severity in POAG patients.
Project description:Globally, irreversible vision loss is mainly attributed to glaucoma, a complex eye condition. Given that the progression of glaucoma is challenging to detect and track, and reliable predictive markers are lacking, there is an urgent need for biomarkers. In this context, the aqueous humor, which is part of the anterior segment of the eye, assumes a critical role. The aim of this study is to develop a comprehensive database for glaucoma research by analyzing primary open-angle glaucoma (POAG) human aqueous humor samples (n=66). We employ advanced label-free quantitative proteomics to investigate the proteome associated with glaucoma in human aqueous humor. Our dataset, generated using the TimsTOF™ Pro Bruker mass spectrometer and DIA-NN bioinformatics for library-independent quantification of data-independent acquisition (DIA) proteomics, offers researchers access to the most extensive catalog of human aqueous humor proteins reported to date. This resource is expected to deepen our understanding of the proteomic profiles in human glaucoma aqueous humor and also support investigations into proteomes related to other ocular conditions.
Project description:This study aimed to investigate the molecular mechanism responsible for primary open-angle glaucoma (POAG) progression. We analyzed microRNAs (miRNAs) expression profiling in aqueous humor (AH) of both POAG patients and normal controls, using a microarray-based approach. Subsequently, differentially expressed miRNAs (DEmiRNAs) were identified using Bayes moderated t-test. Next, DEmiRNAs target genes were predicted based on miRNA databases, followed by GO analysis and pathway analysis using DAVID. Furthermore, OAG-related genes analysis for target genes was carried out using CTD database, respectively. Finally, verification of DEmiRNAs expression levels was performed by RT-qPCR. A total of 40 significant DEmiRNAs were identified between control and POAG groups, including 24 up-regulated miRNAs and 16 down-regulated miRNAs. Further, the target genes of hsa-miR-206, including BMP2, SMAD4, ID2, and TNF, were mainly enriched in transforming growth factor-β (TGF-β) signaling pathway. While, target genes of hsa-miR-184, hsa-miR-34c-5p, hsa-miR-7-2-3p and hsa-miR-20b-3p, including BCL2, EPHB2, VEGFA, COL4A1, APC, and TGFBR1, were enriched in eye development. Moreover, FNDC3B, CAV2 and VEGF, target genes of hsa-miR-206 or hsa-miR-34c-5p, were the OAG-related genes. Ultimately, RT-qPCR analysis confirmed that mRNA levels of hsa-miR-206, hsa-miR-7-2-3p, and hsa-miR-20b-3p were increased, while those of hsa-miR-184 and hsa-miR-34c-5p were decreased in POAG compared with normal groups (P < 0.05). Hsa-miR-206, hsa-miR-184, hsa-miR-34c-5p, hsa-miR-7-2-3p and hsa-miR-20b-3p might play a significant role in the pathogenesis of POAG and hsa-miR-206 might be associated with the development of POAG by regulating TGF-β signaling pathway. These results might provide insight toward a better understanding of the pathogenesis of POAG.