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Mus musculus

ABSTRACT: Next Generation sequencing and quantitative transcriptomic analysis in -77+/+ and -77-/- erythroid precursors

PROVIDER: PRJNA378680 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5331880.fastq.gz	Fastqsanger.gz
	SRR5331881.fastq.gz	Fastqsanger.gz
	SRR5331882.fastq.gz	Fastqsanger.gz
	SRR5331877.fastq.gz	Fastqsanger.gz
	SRR5331878.fastq.gz	Fastqsanger.gz

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Next Generation sequencing and quantitative transcriptomic analysis in -77+/+ and -77-/- erythroid precursors

Project description:Gene expression profiles of sorted erythroid precursors from E14.5 wild-type (WT) and -77 enhancer knockout (-77-/-) mouse fetal liver were generated by next generation sequencing, in triplicate, using Illumina Hiseq2500 (1X50). We showed that the Gata2 -77 enhancer generated the vital erythroid precursor Burst Forming Unit-Erythroid by two mechanisms, GATA-1 upregulation to establish the erythroid transcriptome and GATA-2-dependent, GATA-1-independent transcriptional regulation.

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