Project description:The genes induced by mechanical stimuli may be also involved in disease resistance and wood formation and development in Acacia koa. If so, mechanically stressed A. koa may be used as a model to study disease resistance and wood formation and development. Microarray analysis was performed to determine expression levels of 4,000 genes related to disease resistance and wood development in Acacia koa in response to mechanical stimuli (touch).
Project description:This study investigates the molecular pathogenesis of Ceratocystis manginecans, a fungal pathogen responsible for wilt and canker disease in Acacia mangium. Using a TMT-based quantitative proteomics approach, protein expression was profiled at early stages (1 h and 24 h post-inoculation) following infection with two fungal isolates, CAM and CEP. A total of 205 proteins were identified, with 105 differentially expressed across conditions. The results highlight key virulence-related processes such as DNA repair, oxidative stress response, and host-specific metabolic adaptation. This dataset reveals valuable protein-protein interaction networks that underpin pathogenicity and may inform strategies for disease control in forest plantations.
Project description:Nectaries are the glands responsible for nectar secretion. In the tree Acacia cornigera, extrafloral nectaries produce nectar primarily consumed by mutualistic ant colonies. To understand the genetic programming underlying nectar production, and in particular the connection of the jasmonic acid pathway with the mutualistic ants, extrafloral nectaries tissue was collected at ten time points and conditions, with RNA being isolated and subjected to Illumina RNA-seq analysis.
