Project description:UM1 and UM2 are paired cell lines generated from a oral cancer patient [Nakayama, et al Invas. Metast., 18 (1998), pp. 219–228]. UM1 is highly invasive and exhibits elevated metastatic potential as compared to UM2.
Project description:Ornithine decarboxylase antizyme 1 mediates DNA demethylation. Using a human oral cancer cell line, UM1, genes induiced by DNA demethylation were screened. To verify the methylation status of those induced genes, the gene expression profile was compared with that of 5-Aza-2’-deoxycytidine treated sample. Keywords: DNA demthylation, ornithine decarboxylase antizyme 1, 5-Aza-2’-deoxycytidine
Project description:High glucose has a significant effect on cancer progression. We aim to investigate the effect of glucose on oral cancer cells at gene expression level. We use microarray to identify differentially expressed gene in oral cancer cells (UM1) that cultured in different glucose concentration.
Project description:Ornithine decarboxylase antizyme 1 mediates DNA demethylation. Using a human oral cancer cell line, UM1, genes induiced by DNA demethylation were screened. To verify the methylation status of those induced genes, the gene expression profile was compared with that of 5-Aza-2â-deoxycytidine treated sample. Preparation of samples, hybridization process and data analysis were performed according to the manufacture's protocol (BD Biosciences). To verify the genes induced by ornithine decarboxylase antizyme via DNA demethylation, 5-Aza-2â-deoxycytidine treatment was carried out as a parallel experiment.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
