Project description:High glucose has a significant effect on cancer progression. We aim to investigate the effect of glucose on oral cancer cells at gene expression level. We use microarray to identify differentially expressed gene in oral cancer cells (UM1) that cultured in different glucose concentration.
Project description:UM1 and UM2 are paired cell lines generated from a oral cancer patient [Nakayama, et al Invas. Metast., 18 (1998), pp. 219–228]. UM1 is highly invasive and exhibits elevated metastatic potential as compared to UM2.
Project description:Ornithine decarboxylase antizyme 1 mediates DNA demethylation. Using a human oral cancer cell line, UM1, genes induiced by DNA demethylation were screened. To verify the methylation status of those induced genes, the gene expression profile was compared with that of 5-Aza-2’-deoxycytidine treated sample. Keywords: DNA demthylation, ornithine decarboxylase antizyme 1, 5-Aza-2’-deoxycytidine
Project description:Ornithine decarboxylase antizyme 1 mediates DNA demethylation. Using a human oral cancer cell line, UM1, genes induiced by DNA demethylation were screened. To verify the methylation status of those induced genes, the gene expression profile was compared with that of 5-Aza-2â-deoxycytidine treated sample. Preparation of samples, hybridization process and data analysis were performed according to the manufacture's protocol (BD Biosciences). To verify the genes induced by ornithine decarboxylase antizyme via DNA demethylation, 5-Aza-2â-deoxycytidine treatment was carried out as a parallel experiment.
Project description:Breast cancer line MDA-MB-231 was cultured in complete culture medium containing high glucose DMEM (4.5 g/L) supplemented with 10% fetal bovine serum, 2 mM L-glutamine and 1% antibiotic-antimycotic solution ( Gibco) in an MCO-18AC incubator (Sanyo, Japan) at 37ºC and 5% CO2 concentration in the air. Cell reseeding was carried out every 2-3 days according to the standard protocol. The protocol for obtaining the MDA-MB-231 cell line with stable knockdown of IGFBP6 was described previously (Nikulin et al., 2021).
Project description:To analyze gene expression profiles of differentiated and undifferentiated cancer cells, oral squamous cell carcinoma cell line HSC-2 cells were cultured under normal or adhesion-restricted condition and subjected to cDNA microarray analysis.
Project description:This project was aimed to study the transciptomic profiles of cholangiocarcinoma cells cultured in different concentration of glucose. The established human cholangiocarcinoma cell line; KKU-213, and highly metastatic subline; KKU-213L5, were used. KKU-213 were cultured in either Dulbecco Modified Eagle's Medium (DMEM) with normal (5.6 mM) or high glucose (25 mM) and labeled as KKU-213NG or KKU-213HG according to thier cuture medium and KKU-213L5 was cultured in high glucose DMEM medium. The RNA from all conditions (KKU-213NG, KKU-213HG and KKU-213L5) were then extracted and quality-controlled. Qualified RNA were subjected to sequenced and transcriptomics were processed for the abundance of genes expresed in each condition. The differentially expressed genes were then selected for furhter study to figure out the interaction between them and also the candidate genes that might be a promising target for the treatment of cholangiocarcinoma patients who also have diabetes mellitus.
Project description:Intervention1: Biopsy: The biopsy tissue will be processed to obtain the primary cell line. In the next stage of the study, the cultured primary cell line will be subjected to
various chemotherapeutic agents in different concentrations and studied using real
time cell analyzer for 5 days to obtain IC50 values. Both the primary cells and
biopsy sample will be subjected to microarray, qPCR and proteomics.
Control Intervention1: Nil: Nil
Primary outcome(s): To validate methodology to establish primary tumor cell line from biopsy samples of
cancer patients like breast cancer, NSCLC, Head & Neck, Colorectal.Timepoint: Baseline
Project description:MCF7 is an epithelial cell line isolated from the breast tissue of a patient with metastatic adenocarcinoma. Whether glucose autoregulates its own metabolism remains poorly understood. To probe this, we performed RNA-Seq analysis on MCF7 breast cancer cells cultured with/without short-term glucose withdrawal.
