Project description:To determine te circular expression profile in collagen-induced arthritis (CIA) rats and normal rats, we uesed Arraystar Rat circRNA Array to examine the expression of circRNAs in CIA and normal rats' synovial tissues.
Project description:To determine te lncRNA expression profile in collagen-induced arthritis rats and normal rats, we uesed lncRNA microArray analysis form Arraystar to examine the expression of lncRNAs in CIA and normal rats' synovial tissues.
Project description:Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial inflammation, immune cell infiltration, and progressive joint destruction. Commonly used mouse models each carry practical constraints: collagen-induced arthritis (CIA) is largely restricted to the DBA/1 strain, whereas collagen antibody-induced arthritis (CAIA) is limited by antibody cost. To combine strain flexibility with a robust and reproducible effector phase, we established collagen- and antibody-induced arthritis (C&AIA) on the C57BL/6 background. To characterize the synovial cellular landscape of this model, we performed droplet-based single-cell RNA sequencing on synovial tissue dissected from inflamed knee joints of arthritic C&AIA mice. This dataset resolves the immune and stromal compartments of the C&AIA synovium and enables cross-species comparison with human RA synovial single-cell data.
Project description:To examine patterns of gene expression in ankle synovial fluid cells and peripheral blood leukocytes during serum transferred arthritis. A time-course microarray analysis of serum-transferred arthritis was performed, examining ankle tissue, synovial fluid, and peripheral blood leukocytes.
Project description:The destruction of bone and cartilage results in a loss of joint functionality, critically impairing the quality of life in arthritis patients. Synovial fibroblasts (SFs) critically contribute to the pathogenesis of rheumatoid arthritis (RA) by acquiring either a pro-inflammatory or tissue-destructive phenotype. To explore the molecular mechanisms underlying the pathogenic fibroblast phenotype in arthritis, we performed single-cell RNA sequencing (scRNA-seq) on the synovial cells which were isolated from collagen-induced arthritis (CIA) mice.
