Project description:D is an abundant RNA modification found in all kingdoms of life; yet our understanding of this modified base in mammals is lacking due to the lack of detecting methods. Until now, even for the most abundanct RNA speices with D, tRNA, we still do not have the profile of its sites. In this work, we introduce Chemical Reduction Assisted Cytosine Incorporation sequencing (CRACI), a powerful method that enables whole-transcriptome quantitative mapping of D modifications at single-base resolution.
Project description:Here we studied genome-wide localization of Gcn5 under normal and KCl stress conditions in both yeast species. We found that in Saccharomyces cerevisiae, the enrichment of Gcn5 on genes changes from a relatively even distribution between coding region and intergenic region in the absence of stress, to a predominant localization in gene coding regions under stress conditions. This altered pattern changes are at global level indicates an important role of Gcn5 in modifying chromatin structure for stress adaptation in S. cerevisiae. The altered pattern changes are not observed in Saccharomyces pombe suggesting the different regulatory mechinery between two yeast speices. The related data series is GSE5218, where we have compared the gene regulation of Gcn5 at expression level in S. cerevisiae and S. pombe.
