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ABSTRACT: e9.5 Placenta RNAseq IMB-ASTAR 2017

PROVIDER: PRJNA391361 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5738150.fastq.gz	Fastqsanger.gz
	SRR5738151.fastq.gz	Fastqsanger.gz
	SRR5738152.fastq.gz	Fastqsanger.gz
	SRR5738153.fastq.gz	Fastqsanger.gz
	SRR5738154.fastq.gz	Fastqsanger.gz

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Integrating ATAC-seq and RNA-seq reveals tissue-specific genes and repressed networks in the placenta

Project description:We performed ATAC-seq on placenta dissected from e9.5 timed-pregnant mice in order to generate genome-wide chromatin accessibility maps. Integrating this data with previously published RNA-seq data showed that: 1) genes that have high expression and high ATAC-seq read coverage at the transcription start site (TSS) were enriched for house-keeping functions; 2) genes that have high expression and medium-low coverage at the TSS were enriched for placenta-specific genes and functions; 3) genes that have low expression and high TSS coverage are actively repressed. From the actively repressed genes, we extracted a protein-protein interaction network enriched for neuronal functions. These data increase our understanding of active and repressed gene networks in the e9.5 placenta. The analysis also provides a method for identifying repressed networks and tissue-specific genes by integrating ATAC-seq and RNA-seq data. Please note that the RNA-Seq data are already deposited and available under GSE65808.

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