Project description:We have performed RNA sequencing on kidneys from inclusion body nephropathy-affected mice and compared the data to healthy, uninfected controls. Using a metagenomics approach, we report the identification of the disease causing agent as an atypical virus, mouse kidney parvovirus (MKPV), belonging to a divergent genus of the Parvoviridae. The RNA sequencing also enabled us to assess the host response to MKPV-infection.
Project description:We have developed a generally adaptable, novel high-throughput chromosome conformation capture assay for use in trans (V3C-seq) that allows genome-wide identification of the direct associations of a lytic virus genome with discreet regions of the cellular chromosome. Upon infection, the parvovirus Minute Virus of Mice genome associated directly with sites of cellular DNA damage. These sites also exhibited damage in uninfected cells when cycling through S-phase. As infection proceeded, new sites of DNA damage were induced, and virus subsequently also associated with these.
