Project description:It has been demonstrated that parvovirus infection causing cytotoxicity and immune activation effect in many virus-induced cell lines, however, the underlying mechanisms are not fully understood. To investigate the gene expression signature of host cell after CPV-2 infection, we performed transcriptome profiling of MDCK after persistent infection of CPV-2a using microarray analysis. Uninfected MDCK cells act as negative control. The data show the genes regulated by CPV-2 infection..
Project description:We utilized high-throughput small RNA-seq technology to investigate the altered miRNA profiling in miRNA libraries from uninfected (Control) and canine parvovirus type 2c (CPV-2c) infected Crandell Reese Feline Kidney (CRFK) cells. We identified five of known miRNAs (miR-222-5p, miR-365-2-5p, miR-1247-3p, miR-322-5p, and miR-361-3p) and 3 novel miRNAs (Novel 137, Novel 141, and Novel 102) by small RNA-seq with differentially expressed genes (DEGs) in the miRNA repertoire of CPV-infected cells over control. We further predicted the potential target genes of aforementioned miRNAs using sequence homology algorithms. Notably, the targets of miR-1247-3p exhibited a potential function associated with cellular defense and humoral response to CPV. We discovered 229 putative targets from a total of 38 enriched GO terms. We next constructed GO biological process network composed of 28 target genes of miR-1247-3p, of which, some genes, namely BCL6, DLL1, GATA3, IL6, LEF1, LFNG, and WNT1 were among the genes with obviously intersected in multiple GO terms. The miRNA-1247-3 and its cognate target genes suggested the great potential as novel therapeutic targets or diagnostic biomarkers of CPV or other related viruses.
Project description:Canine parvovirus (CPV) infection poses a serious threat to pangolins, but the characteristics of the host response at the metabolic level remain unclear. In this study, we used non-targeted fecal metabolomics to systematically analyze differences in fecal metabolites in the Malaysian pangolin and the Chinese pangolin before and after CPV infection. The results indicated that CPV infection significantly altered the fecal metabolic profiles of both pangolin species. In the Malaysian pangolin, post-infection differential metabolites were primarily enriched in lipid metabolic pathways, such as α-linolenic acid and arachidonic acid metabolism, as well as inflammation-related pathways, including ferroptosis and necrotic apoptosis. Whereas the Chinese pangolin exhibited not only lipid metabolism dysregulation but also amino acid metabolic reprogramming and bile acid metabolism dysregulation. There were significant differences in metabolic responses between the two species, with the Malaysian pangolin characterized by lipid metabolism abnormalities, while the Chinese pangolin showed a greater focus on amino acid and bile acid metabolism. This study revealed the specific metabolic response of pangolins infected with CPV, provided potential fecal metabolic biomarkers for the early identification of CPV infection in pangolins, and provided a new theoretical basis for disease monitoring and intervention in pangolin conservation medicine.