Project description:Precise species detection of traditional herbal patent medicine Qingguo Wan by shotgun metabarcoding Genome sequencing and assembly
| PRJNA662455 | ENA
Project description:Precise species detection of traditional Chinese patent medicine
| PRJNA529027 | ENA
Project description:Shotgun metabarcoding of traditional herbal products
Project description:Herbal preparations are complex mixtures of natural products, many of which are able to reach the distal gut. There, they influence the microbial communities, and can be metabolized into bioactive metabolites. The aim of this study was to establish an interdisciplinary platform to unravel interactions of herbal medicine and intestinal microbiota, using a combined LC-MS metabolomics and 16S rRNA microbiome sequencing approach. Willow bark extract (WBE), a herbal medicinal product with well-established anti-inflammatory activity, was incubated with human fecal suspension under anoxic conditions. Samples were drawn after 0.5, 4 and 24 h of incubation.</p>Microbiome analyses revealed that incubation with WBE had a marked effect on microbial community composition and functions. In particular, the proportion of Bacterioides sp., generally regarded as beneficial for the gut ecosystem, was clearly enhanced in WBE-treated samples. LC-MS analysis showed that WBE constituents were readily metabolized by fecal bacteria. Numerous microbial metabolites could be annotated, allowing the construction of putative microbial degradation pathways for the main groups of WBE constituents.</p>We suggest that studies of this type help to increase the knowledge on bioactive principles of herbal medicine, since gut microbial metabolites have certainly been underestimated as a source of bioactive compounds in the past.
Project description:Abstract Background:Colorectal Cancer (CRC) is a common malignant tumor of the digestive tract, with poor prognosis in advanced stages due to metastasis and immune evasion.Chronic intestinal inflammation may promote CRC progression by inducing an imbalance in the immune microenvironment, thus therapeutic strategies targeting immune regulation urgently need to be explored.Wumei Wan (WMW), a traditional Chinese herbal formula, has shown marked anti-inflammatory activity in ulcerative colitis (UC).However, whether it can inhibit CRC progression remains unclear. Aim:This study aims to investigate whether WMW can ameliorate CRC and to explore its underlying mechanisms. Methods:An AOM/DSS-induced CRC mouse model was established, and transcriptomicss, metabolomics, and network pharmacology analyses were integrated to investigate and validate the mechanisms of WMW. Results:WMW ameliorated CRC progression by improving colonic pathological conditions, lowering Disease Activity Index (DAI) scores, enhancing survival, and suppressing tumor growth in CRC mice. Specifically, the WMW group’s DAI score decreased by approximately 34.36% compared to the model group. Additionally, by day 70, the survival rate in the WMW-treated group was 22.806% higher than that of the model group. Multi-omics and network pharmacology analyses indicated that WMW inhibited the IDO-Kyn-AHR signaling pathway, decreased Th17 and Treg cell proliferation and differentiation, restored CD8+T cell function, and enhanced antitumor immunity. Conclusion：This research demonstrates for the first time that WMW suppresses CRC progression by rebalancing Th17/Treg immunity through modulation of the IDO1-Kyn-AHR pathway.
Project description:Our study provides detailed analysis of DF-induced transcriptomes generated by RNA-Seq technology. RNA-Seq based transcriptome analyzes would elucidate complex molecular mechanisms of multi-herbal composition like DF
Project description:Asterids is one of the major plant clades comprising of many commercially important medicinal species. One of the major concerns in medicinal plant industry is adulteration/contamination resulting from misidentification of herbal plants. This study reports the construction and validation of a microarray capable of fingerprinting medicinally important species from the Asterids clade.
Project description:Ginkgo biloba leaf extract (GBE) has been used for centuries in traditional Chinese medicine and today is used as an herbal supplement for various indications such as improving neural function, anti-oxidant and anti-cancer effects. As part of the herbal supplement industry, these compounds are largely unregulated, and may be consumed in large concentrations over extended periods of time. This is of particular concern, because the long-term effects in terms of toxicity and carcinogenicity data is lacking for many herbal products, including GBE. The 2-year B6C3F1 mouse carcinogenicity bioassay indicated a marked dose-related increase in hepatocellular carcinoma (HCC) development associated with exposure to GBE. We have shown that the mechanism of this increase in tumorigenesis is related to a marked increase in the incidence of β-catenin mutation, and report a novel mechanism of constitutive β-catenin activation through post-translational modification leading to constitutive Wnt signaling and unregulated growth signaling and oncogenesis. Furthermore, using global gene expression profiling, we show that GBE-induced HCC exhibit overrepresentation of gene categories associated with human cancer and HCC signaling including upregulation of relevant oncogenes and suppression of critical tumor suppressor genes, as well as chronic oxidative stress, a known inducer of calpain-mediated degradation and promoter of hepatocarcinogenesis in humans. These data provide a molecular mechanism to GBE-induced HCC in B6C3F1 mice that is relevant to human cancer, and provides relevant molecular data that will provide the groundwork for further risk assessment of unregulated compounds, including herbal supplements. Six hepatocellular carcinomas induced by GBE, six spontaneous hepatocellular carcinomas, and six normal liver samples, three technical replicates each.
Project description:Although patients of colorectal cancer use Traditional Chinese Medicine (TCM) herbal therapy extensively in China, no strong evidence exists to demonstrate the safety and survival outcomes of TCM herbal therapy combined with conventional western medicine for treatment of this disease. The purpose of this multi-center perspective cohort study is to evaluate the relationship between TCM herbal therapy and survival outcomes in patients with advanced colorectal cancer.
