Project description:Compared to wildtype macrophages, IRAK2 deficient macrophages show higher induced gene expression in responsse to CpG B, but not R848 Manuscipt title: The dual function of IRAK2 in TLR9-mediated interfereon and proinflammatory cytokine production Bone marrow derived macrophages from wildtype and IRAK2 knockout mouse were stimulated with CpG B or R848 for 2 hours, or untreated.
Project description:We previously identified TLR-independent expression of 4933430F08Rik, encoding Batf2, in T. cruzi-infected bone marrow-derived dendritic cells (BMDCs) (Kayama et al., 2009). To determine the functions of Batf2 in innate immune responses, we performed a comprehensive gene expression analysis in wild-type and Batf2-/- bone marrow-derived macrophages (BMMφ). RNA-seq analysis showed that 98 genes are upregulated in Batf2-/- BMMφ stimulated with LPS following IFN-γ treatment, when compared with that in wild-type cells. Among these genes, we focused on Il23a, encoding IL-23p19, because IL-23 is able to promote expression of Il17a in Th17 cells.
Project description:To understand the molecular mechanism underlying the miR192-mediated modulation of inflammatory response, we have employed whole genome microarray expression profiling of bone-marrow derived macrophages transfected with miR192. In order to distingish the effect of miR192 on inflammatory response from the effecct of IL-6, RNA was isolated from miR192-transfected and R848-stimulated macrophages in the presence or absence of anti-IL-6 Ab during the culture.
Project description:To further understanding the function of cullin 3 during inflammation in macrophages, we have employed mouse bone marrow derived macrophages microarray expression profiling to identify the gens that involve in regulationg inflammatory respones upon LPS challenge. Mouse BMM were stimulated with LPS for 6 hours and RNA was extracted for microarray. Ogt was indentified by comparison between wildtype and Cullin 3 knockout BMM.
Project description:Using microarrays, we compared the changes in levels of gene expression between wildtype and Bcl6 KO macrophages in the absence or presence of LPS. Total RNA was obtained from WT and Bcl6 KO unstimulated and LPS-stimulated primary bone marrow-derived macrophages
Project description:Bone marrow derived macrophages (BMDM) generated from c57bl/6j mice bone marrow cells were stimulated for 18 h with 12 microgram/ml adiponectin, RNA from non-stimulated or 18 h adiponectin-stimulated BMDM subjected to a agilent microarray analysis
Project description:Itaconate is a natural mild electrophile, able to modify free thiols in proteins upon activation. We submit two datasets investigating peptides modified by itaconate. Experiment001 contains data obtained from endogenous itaconate producing WT or itaconate incompetent Irg1-/- Bone marrow-derived macrophages stimulated with LPS for 24h. The second dataset from Experiment002 contains data obtained from WT Bone marrow-derived macrophages treated with media or 5 mM itaconic acid for 16h.
Project description:Transcriptional profiling of mouse bone marrow derived macrophages comparing wildtype with CDK5R1 knockout. CDK5R1 is an activating parter for CDK5 and synthesized upon TLRs stimaultion. Activated CDK5 has various target substrates, and functions as a key singnaling regulator in macrophages. Two condition experiments: basal state and LPS-stimulated macrophages for 6 hours. Two biological replicates.
Project description:We assessed the role of basic leucine zipper transcription factor ATF-like 2 (Batf2), particularly its positive transcriptional activities via TLR signals. Because TLR7 agonists are clinically used against tumors and have proven effective as antitumor drugs, we assessed effect of Batf2 on the responses to the TLR7 ligand (R848).
