Project description:We sequenced the genomes of 32 isofemale fly lines from two divergent microclimates at 'Evolution Canyon' in Israel (16 fly lines from each microclimate).
Project description:We performed RNA-seq to profile gene expression in the heads and whole bodies of 32 isofemale fly lines from two divergent microclimates at 'Evolution Canyon' in Israel (16 fly lines from each microclimate). We also measured RNA editing levels in the head tissue of these flies.
Project description:Bulk ATAC-seq performed on whole adult brains across multiple homozigous fly lines (DGRP) in order to find caQTLs. Young adults (1-3 days) were used for all genotypes.
Project description:Whole-genome sequencing in inbred (D907) and outbred (Mix) fly populations from lines selected for the ectopic veins trait under genetic assimilation (assimilated) or direct artificial selection under normal conditions (non-assimilated), unselected (control) and ancestral (P0) fly populations.
Project description:The Personalized Discovery Process is the only program offering patients treatment recommendations based on an empirically constructed Drosophila "fly" model of their disease. Special committee selects one of the one of the few 2-3 FDA approved drug combinations or single agents that improved survival in the fly cancer model.
Project description:Profiling of histone modifications with the iChIP protocol (PMID25103404) using antibodies against two markers of regulatory activity: H3K27ac (abcam, ab4729) and H3K4me3 (Millipore, 07-473). All paternal fly lines were taken from the Drosophila Genetic Reference Panel crossed to a common mother (PMID31308546). Data were collected at three time points (2-4h, 6-8h, 10-12h at 25C) with two biological replicates per collection.
Project description:CUT&RUN assays in inbred (D907) and outbred (Mix) fly populations from lines selected for the ectopic veins trait under genetic assimilation (assimilated) or direct artificial selection under normal conditions (non-assimilated) and unselected (control) flies.
Project description:Hi-C experiments on wing discs (WD) from D907 inbred fly populations from lines selected for the ectopic veins trait by genetic assimilation (assimilated) or direct artificial selection under normal conditions (non-assimilated) and unselected (control) flies.
Project description:To measure the response to gene dose, we performed mRNA-Seq of fly heads with molecularly defined deletions constructed from DrosDel deficiency lines (Ryder et al. Genetics 2007, 177(1):615-29) on the Illumina HiSeq 2000 platform.