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Ovis aries

ABSTRACT: α7 nicotinic acetylcholine receptor signaling modulates the inflammatory phenotype of fetal brain microglia: first evidence of interference by iron homeostasis.

PROVIDER: PRJNA395718 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5865700.fastq.gz	Fastqsanger.gz
	SRR5865691.fastq.gz	Fastqsanger.gz
	SRR5865701.fastq.gz	Fastqsanger.gz
	SRR5865692.fastq.gz	Fastqsanger.gz
	SRR5865702.fastq.gz	Fastqsanger.gz

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α7 nicotinic acetylcholine receptor signaling modulates the inflammatory phenotype of fetal brain microglia: first evidence of interference by iron homeostasis.

Project description:We sequenced mRNA from 7 microglia extracted from sheep fetuses exposed to LPS, agonist and antagonist of α7 nicotinic acetylcholine receptor to determine signaling pathway through modeling of α7 during inflammation.

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α7 Nicotinic Acetylcholine Receptor Regulates Macrophage-Mediated Neuroinflammation in Dry Eye Disease

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Actication of alpha7 nicotinic receptor regulates herpes simplex virus infection in the central nervous system

Project description:mRNA isolated from a mouse microglia cell line, BV2 cells, treated with or without α7 nicotinic receptor agonist, choline, before HSV-1 infection. mRNA expression was examined with the NanoString© platform (www.nanostring.com) utilizing the Mouse Neuropatholgy codeset (NS_Mm_neuropath_V1.0) that measures 760 endogenous and 10 housekeeping (HK) RNAs.

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Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1).

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Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1) and performed a detailed survey of gene expression across different tissues. RNA-seq data of 7 tissue types from the reference female Texel and skin tissue from a Gansu alpine fine wool sheep were sequenced.

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Ovis aries

Project description:Expression profiling of prion accumulating ovine microglia

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α7 nicotinic acetylcholine receptor signaling modulates fetal sheep brain astrocytes transcriptome in response to endotoxin stress: comparison to microglial transcriptome and implications for autism spectrum disorder

Project description:Neuroinflammation in utero may result in lifelong neurological disabilities. Astrocytes play a pivotal role, but the mechanisms are poorly understood. No early postnatal treatment strategies exist to enhance neuroprotective potential of astrocytes. We hypothesized that agonism on α7 nicotinic acetylcholine receptor (α7nAChR) in fetal astrocytes will augment their neuroprotective transcriptome profile, while the antagonistic stimulation of α7nAChR will achieve the opposite. Using an in vivo - in vitro model of developmental programming of neuroinflammation induced by lipopolysaccharide (LPS), we validated this hypothesis in primary fetal sheep astrocytes cultures re-exposed to LPS in presence of a selective α7nAChR agonist or antagonist. Our RNAseq findings show that a pro-inflammatory astrocyte phenotype acquired in vitro by LPS stimulation is reversed with α7nAChR agonistic stimulation. Conversely, antagonistic α7nAChR stimulation potentiates the pro-inflammatory astrocytic phenotype. Furthermore, we conduct a secondary transcriptome analysis against the identical α7nAChR experiments in fetal sheep primary microglia cultures and against the Simons Simplex Collection for autism spectrum disorder and discuss the implications.

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