Project description:The assess the effect of AvrSr35 knock-out on host-pathogen interaction in the compatible host, we have performed time-course analysis of leaf transcriptomes obtained by infecting susceptible wheat cultivar Fielder with the wild type Puccinia graminis f.sp. tritici (Pgt) isolate 99KS76A-1 and its three mutants, M1, M4 and M7, that carry loss-of-function mutations in the AvrSr35 gene.
Project description:This study presents transcriptome profiles of multiple tissues (root, stem, leaf, flower, and pod) from Vigna radiata var. sublobata, a wild relative of cultivated mung bean. RNA sequencing was performed using Illumina platform to investigate tissue-specific gene expression patterns. The data will facilitate understanding of gene regulation in different plant organs and provide resources for comparative genomics with cultivated Vigna radiata. Experimental design: Tissues were collected from mature plants grown under controlled conditions. Three biological replicates per tissue type. Libraries were prepared using poly-A selection and sequenced in paired-end mode (2x150 bp). This submission includes raw sequencing data (FASTQ files) for all samples.