Project description:The Fusarium incarnatum strain K23, originally isolated from a habit-adapted wild plant Thapsia species, colonized the roots and shoots of tomato seedlings and protected them against salt stress. Comparison of expression and metabolite profile changes uncovered that the fungus completely reprogramed the tomato response to salt stress. Barely any overlap was observed among the genes and metabolites which are regulated by salt stress in uncolonized and colonized tomato seedlings. In colonized seedlings exposed to salt stress, less stress- related genes are activated than in un-colonized seedlings. Furthermore, K23 produced gibberellin and gibberellin-responsive genes were detected in all RNA samples. Our analysis demonstrates that K23 colonisation completely alters the salt-responsive gene and metabolite profiles in tomato seedlings.
Project description:Impact of silicon supply on accumulation of proteins from Trifolium incarnatum L and from Rhizobium leguminosarum bv trifolii (T354) in nodules of Trifolium incarnatum L cultivated under nitrogen deficiency conditions. For each modality, the nodules were separated from the root and their proteins extracted and analysed by mass spectrometry. The proteins of each species were analysed separately, and Trifolium proteins were used for GO enrichment, while Rhizobium proteins were classified manually using their Uniprot functions (due to the absence of a specific database).
