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Populus trichocarpa cultivar:Nisqually-1

ABSTRACT: Gene expression analysis of Populus trichocarpa Nisqually-1 - mature leaf - 076-G07 transcriptome

PROVIDER: PRJNA402523 | ENA |

REPOSITORIES: ENA

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Populus trichocarpa cultivar:Nisqually-1

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High resolution survey of drought, high salinity, heat and cold stress-induced transcriptome of bioenergy feedstock model plant western poplar (Populus trichocarpa) accession Nisqually-1

Project description:Abiotic stress is a major factor affecting the growth, development, yield and quality in plants including the important biomass yield such as in a bioenergy feedstock crops. In a first of its kind, RNA-seq based high resolution survey of abiotic stress-induced transcriptome of bioenergy feedstock model plant western poplar (Populus trichocarpa accesion Nisqually-1) was carried out by way of 81 libraries made from total RNA isolated from three tissues, mature vascular leaf, stem xylem and root sampled from plants subjected to untreated control and treated with four individual stress treatments cold, heat, drought and high salinity. For every tissue and treatment type including controls, three individual plants were used per treatment as biological replicates. This research project is supported by the DOE Office of Science, Office of Biological and Environmental Research (BER), USA, grant no. DE-SC0008570

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Project description:Populus trichocarpa Nisqually-1 Transcriptome - 076-I08

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Ozone exposure of Populus deltoides and Populus trichocarpa

Project description:Populus deltoides and Populus trichocarpa were exposed to either ambient air or an acute ozone exposure of 200 ppb for 9 hrs and ozone response was profiled for each genotype by hybridising control against ozone-exposed samples per genotype. Keywords: stress response, genotype comparrison, ozone exposure RNA was extracted from the fifth leaf below the first fully unfurled leaf for each plant. Control and ozone-exposed plants were then randomly paired for hybridisation.

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Ozone exposure of Populus deltoides and Populus trichocarpa

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