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Changes in H3K27ac following LPS stimulation in pharyngeal epithelial cells

ABSTRACT: Changes in H3K27ac following LPS stimulation in pharyngeal epithelial cells

PROVIDER: PRJNA413376 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6132670.fastq.gz	Fastqsanger.gz
	SRR6132671.fastq.gz	Fastqsanger.gz
	SRR6132664.fastq.gz	Fastqsanger.gz
	SRR6132665.fastq.gz	Fastqsanger.gz
	SRR6132666.fastq.gz	Fastqsanger.gz

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Changes in H3K27ac following LPS stimulation in pharyngeal epithelial cells

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Project description:Mouse microglia (BV2 cells) were stimulated with a TLR4 ligand (E. coli LPS, 1 µg/ml) for 4h. The Agilent SurePrint G3 Mouse Gene Expression Microarray (G4852A) was used for the analysis, which provides full coverage of genes and transcripts with the most up-to-date content, including mRNAs and lincRNAs (http://www.chem.agilent.com/store/en_US/Prod-G4852A/G4852A). BV2 cells were grown to 80% confluence for four groups: the siRNA control (Group A, cells treated with a non-specific scrambied siRNA control), the LPS-stimulated (Group B, cells treated with the siRNA control plus LPS stimulation), lincRNA-Cox2 siRNA (Group C, cells treated with an siRNA to lincRNA-Cox2), and lincRNA-Cox2 siRNA/LPS stimulated (Group D, cells treated with the lincNRA-Cox2 siRNA plus LPS stimulation). Cells were treated with the siRNAs for 24h, followed by additional culture for 4h in the presence or absence of LPS (E. coli LPS, 1 µg/ml). Total RNAs were prepared with the RNeasy Mini kit (Qiagen) according to the manufacturer’s instruction (Ambion).

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