Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

BRCA1 haploinsufficient Mesenchyme Derived from human peripheral blood Induced Pluripotent Stem Cell

ABSTRACT: BRCA1 haploinsufficient Mesenchyme Derived from human peripheral blood Induced Pluripotent Stem Cell

PROVIDER: PRJNA413524 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

BRCA1 haploinsufficient Mesenchyme Derived from human peripheral blood Induced Pluripotent Stem Cell

Project description:BRCA1 molecule, known to be implicated in DNA repair and its alterations were associated to familial breast cancer prediposition. Genome of the patients with familial breast cancer predispostion harbor some germinal alterations in BRCA1 locus. Cells from peripheral blood of human with haplodeficiency BRCA1 locus and also from comparative sample (BRCA1 unaffected) were collected to produce induced pluripotent stem cell by classical protocol (Yamanaka OKSM) and Sendai Stem cell reprogammation which was validated by expression assessment of pluripotent stem cell markers and induction of teratoma after xenotransplantation mice model. Induced pluripotent stem cell (IPSC) were differentiated in mesenchymal lineage in order to see influence of BRCA1 effects during developmental steps of the stroma: effectively microenvironment of breast tumor is infiltrate of mesenchymal stromal cell. Total RNA of the mesenchyme derived IPS from control and BRCA1 haploinsufficient were respectively collected to screen whole expression analysis by transcriptome microarray.

2019-01-31 | GSE104693 | GEO

BRCA1 Mutations Attenuate Super-Enhancer Function and Chromatin Looping in Haploinsufficient Human Breast Epithelial Cells

Project description:BRCA1 functions in multiple biological processes, including double-strand break repair, replication stress suppression, transcriptional regulation, and chromatin reorganization. While non-malignant cells carrying cancer-predisposing BRCA1 mutations exhibit increased genomic instability, it remains unclear whether BRCA1 haploinsufficiency affects transcription and chromatin dynamics. Here we show that primary mammary epithelial cells from women with BRCA1 mutations (BRCA1mut/+) display significant loss of H3K27ac-associated super-enhancers.

2019-04-19 | GSE121229 | GEO

BRCA1 Mutations Attenuate Super-Enhancer Function and Chromatin Looping in Haploinsufficient Human Breast Epithelial Cells

Project description:BRCA1 Mutations Attenuate Super-Enhancer Function and Chromatin Looping in Haploinsufficient Human Breast Epithelial Cells

| PRJNA496412 | ENA

Single cell RNA sequencing of mouse indced pluripotent stem cell (iPSC)-derived and mouse primary embryonic lung mesenchyme

Project description:The lung mesenchyme plays important roles in lung development and is affected in many respiratory diseases, yet relatively little is known about the biology of lung mesenchymal progenitors. We sought to establish an induced pluripotent stem cell (iPSC)-based model to study lung mesenchyme development and epithelial-mesenchymal interactions. We generated a mouse iPSC line carrying a lung mesenchyme-specific reporter/tracer to establish a protocol for differentiation into lung mesenchymal progenitors. We derived lung mesenchyme from iPSCs both by directed differentiation via a lateral plate mesodermal progenitor state (induced lung mesenchyme, iLM), and by co-development during lung epithelial differentiation (co-developed lung mesenchyme, cLM). We found that directed differentiation via a lateral plate mesoderm progenitor was not only more efficient, but also yielded engineered lung mesenchymal cells that were more similar to primary lung mesenchyme from day 12.5 mouse embryos, as determined by single cell RNAseq. Our iPSC-derived population will provide an inexhaustible source of cells for studying lung development, modeling diseases, and developing therapeutics.

2023-05-12 | GSE203245 | GEO

BRCA1

Project description:Monodelphis domestica BRCA1, Monodelphis domestica BRCA1 DNA repair associated (BRCA1), mRNA. [Source:RefSeq mRNA;Acc:NM_001033969], is expressed in 2 baseline experiment(s);

| ENSMODG00000015150 | ExpressionAtlas

Brca1

Project description:Rattus norvegicus Brca1, BRCA1, DNA repair associated [Source:RGD Symbol;Acc:2218], is differentially expressed in 25 experiment(s);

| ENSRNOG00000020701 | ExpressionAtlas

Brca1

Project description:Rattus norvegicus Brca1, BRCA1, DNA repair associated [Source:RGD Symbol;Acc:2218], is expressed in 4 baseline experiment(s);

| ENSRNOG00000020701 | ExpressionAtlas

BRCA1

Project description:Macaca mulatta BRCA1, BRCA1 DNA repair associated [Source:VGNC Symbol;Acc:VGNC:99124], is differentially expressed in 1 experiment(s);

| ENSMMUG00000001329 | ExpressionAtlas

BRCA1

Project description:Macaca mulatta BRCA1, BRCA1 DNA repair associated [Source:VGNC Symbol;Acc:VGNC:99124], is expressed in 3 baseline experiment(s);

| ENSMMUG00000001329 | ExpressionAtlas

BRCA1

Project description:Oryctolagus cuniculus BRCA1, BRCA1 DNA repair associated [Source:HGNC Symbol;Acc:HGNC:1100], is expressed in 1 baseline experiment(s);

| ENSOCUG00000003883 | ExpressionAtlas

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data