Project description:Analysis of ovarian cancer cell lines after knockdown of FGFRL1 using SiRNA. To elucidate the signaling pathways that were significantly altered following the silencing of FGFRL1 expression, we performed global gene profiling experiments of the OVCAR8 and ES2 cells after knockdown of FGFRL1 using siRNA. We conducted pathway analysis with the differentially expressed genes using R in two OC cells.
Project description:Cbx7 knockdown resulted in increase of genes related with apoptosis and inflammation. Expression microarray was performed using RNA extracted from 2 ovarian clear cell adenocarcinoma cell lines, namely, KOC-7C and TOV21G, either trancduced by Cbx7 siRNA or control.
Project description:Ascites is an abnormal fluid that accumulates in the peritoneal cavity of more than 90% of patients with metastatic ovarian cancer. Despite its common occurance, little is known about its effects on detached and metastasizing ovarian cancer cells, which frequently metastasize to the peritoneum. This experiment aims to identify any transcriptional changes that occur in ovarian cancer cell lines as a result of exposure to ovarian cancer ascites
Project description:Bulk RNA-seq was performed in ovarian cancer cell lines JHOC-5 and OVCA420 after lentiviral PRSS23 knockdown using two independent shRNAs (shRNA_30 and shRNA_46) or a non-targeting shRNA control. Gene-level read counts for all samples are provided.
Project description:FGFRs regulate PCa development and progression, but the role of the recently found FGFR-like 1 (FGFRL1) remains unclear. The data consists of gene expression profiles of mouse subcutanous xenograft tumors generated of human PC3M prostate cancer cells with stable knockdown of FGFRL1 gene and of the control xenografts.
Project description:DRD2 antagonists are commonly used clinically for the treatment of psychiatric disorders. This study aimed to explore the antitumor effects of DRD2 antagonists on a variety of tumor entities. A DRD2 antagonist, pimozide, exhibited antitumor effects in pancreatic, colorectal, ovarian, and gastric cancers. HMOX1 was found to be highly expressed at both mRNA and protein levels, corroborating the results of profiling. Transcriptional profile of knockdown of HMOX1 or/and pimozide-treated cancer cell lines was showed then.