Ontology highlight
ABSTRACT:
PROVIDER: PRJNA417869 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR6282640_1.fastq.gz | Fastqsanger.gz | |||
| SRR6282640_2.fastq.gz | Fastqsanger.gz | |||
| SRR6282638_1.fastq.gz | Fastqsanger.gz | |||
| SRR6282638_2.fastq.gz | Fastqsanger.gz | |||
| SRR6282639_1.fastq.gz | Fastqsanger.gz |
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Project description:Flower-lotus with many attractive floral characteristics has been studied and discussed the most. These characteristics are used as the standards of the classification in most cases, and always attracted the attention of lotus breeders on improvement program because of associating with ornamental and economic values of lotus. However, molecular mechanisms underlying the formation of these attractive floral features still remain largely unknown. Transcriptome sequencing technique has been established as an efficient approach for gene discovery and expression pattern identification. For some plants, a lot of important genes involved in plant critical metabolisms have been successfully identified by this technique. In the study, mass sequence data obtained from the deep sequencing of a mixed flower-bud cDNA pool from three individuals of N. nucifera provide a platform to comprehensively understand the processes of flower formation and development at the molecular level, and will greatly facilitate the genetic improvement of ornamental characteristics and the directive molecular breeding for lotus in the future.
2014-12-08 | GSE57601 | GEO
Project description:Flower-lotus with many attractive floral characteristics has been studied and discussed the most. These characteristics are used as the standards of the classification in most cases, and always attracted the attention of lotus breeders on improvement program because of associating with ornamental and economic values of lotus. However, molecular mechanisms underlying the formation of these attractive floral features still remain largely unknown. Transcriptome sequencing technique has been established as an efficient approach for gene discovery and expression pattern identification. For some plants, a lot of important genes involved in plant critical metabolisms have been successfully identified by this technique. In the study, mass sequence data obtained from the deep sequencing of a mixed flower-bud cDNA pool from three individuals of N. nucifera provide a platform to comprehensively understand the processes of flower formation and development at the molecular level, and will greatly facilitate the genetic improvement of ornamental characteristics and the directive molecular breeding for lotus in the future. A mixed cDNA pool from young flower-buds (35-40mm in length) of three accessions of N. nucifera were used for deep sequencing using 454 GS-FLX Titanium.
2014-12-08 | E-GEOD-57601 | biostudies-arrayexpress
Project description:Lotus Flower-Bud Transcriptome or Gene expression
| PRJNA246776 | ENA
Project description:Sacred lotus (Nelumbo nucifera) belongs to Nelumbonaceae family. Its seeds are widely consumed in Asia countries as snacks or even medicine. Besides the market values, lotus seed also plays crucial roles in lotus life cycle. Consequently, it is essential to gain a comprehensive understanding on the development of lotus seed. During its development, lotus seed undergoes cell division, expansion, reserve accumulation, desiccation and maturation phases. We observed the morphological and biochemical changes of lotus seed from 10 to 25 days after pollination (DAP) which was corresponding to the reserve synthesis and accumulation phase. The volume of the seed expanded until 20 DAP with the color of the seed coat changing from yellow-green to dark green and gradually faded again. Starch and protein rapidly accumulated from 15 to 20 DAP. To further reveal the metabolism adaptation, primary metabolites and proteins profiles were obtained from the mass spectrometry based platforms. Metabolites and enzymes involved in sugar metabolism, glycolysis, TCA cycle and amino acids metabolism schematized on their biosynthetic pathways. Both metabolic and proteomic profiles indicated more active metabolism from 10 to 15 DAP than after 20 DAP. The results provide a frame of reference for the evaluation of primary metabolism during lotus seed development.
2022-02-22 | PXD003768 | Pride