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The Anaphase Promoting Complex/Cyclosome degrades DMS3 to maintain RNA-directed DNA Methylation in Arabidopsis [small RNA-seq]

ABSTRACT: The Anaphase Promoting Complex/Cyclosome degrades DMS3 to maintain RNA-directed DNA Methylation in Arabidopsis [small RNA-seq]

PROVIDER: PRJNA418705 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6297110.fastq.gz	Fastqsanger.gz
	SRR6297111.fastq.gz	Fastqsanger.gz
	SRR6297112.fastq.gz	Fastqsanger.gz

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The Anaphase Promoting Complex/Cyclosome degrades DMS3 to maintain RNA-directed DNA Methylation in Arabidopsis [lncRNA-seq]

Project description:RNA-directed DNA methylation (RdDM) plays an essential role in transposable element (TE) silencing in plants. In the Arabidopsis RdDM pathway, the DDR complex containing DRD1, DMS3, and RDM1, is necessary for recruiting Pol V to transcribe scaffold RNA. Although the role of DDR is known, the mechanism by which the DDR complex is regulated remains unexplored. Here, we demonstrate that the Anaphase Promoting Complex/Cyclosome (APC/C) monitors the assembly of the DDR complex by targeting DMS3 for degradation. We show that a subset of Pol V-dependent RdDM loci are de-repressed in apc/c mutants, accompanied by defective recruitment and transcription of Pol V. APC/C targets DMS3 for ubiquitination and degradation in a D box-dependent manner, and the D-box-mutated DMS3 fails to complement the dms3 mutant. Competitive binding assays shows that the dosage of DMS3 is critical for the assembly of the DDR complex, and in vivo gel filtration analysis shows that the assembly of both DDR and Pol V is compromised in the apc8 mutant. These findings uncover a safeguard role of APC/C-mediated DMS3 degradation in the assembly of the DDR complex, and provide a direct link between selective protein degradation and RdDM.

2019-01-31 | GSE106970 | GEO

The Anaphase Promoting Complex/Cyclosome degrades DMS3 to maintain RNA-directed DNA Methylation in Arabidopsis

Project description:The Anaphase Promoting Complex/Cyclosome degrades DMS3 to maintain RNA-directed DNA Methylation in Arabidopsis

| PRJNA418691 | ENA

The Anaphase Promoting Complex/Cyclosome degrades DMS3 to maintain RNA-directed DNA Methylation in Arabidopsis [small RNA-seq]

2019-01-31 | GSE106971 | GEO

The Anaphase Promoting Complex/Cyclosome degrades DMS3 to maintain RNA-directed DNA Methylation in Arabidopsis [lncRNA-seq]

Project description:The Anaphase Promoting Complex/Cyclosome degrades DMS3 to maintain RNA-directed DNA Methylation in Arabidopsis [lncRNA-seq]

| PRJNA418706 | ENA

The Anaphase Promoting Complex/Cyclosome degrades DMS3 to maintain RNA-directed DNA Methylation in Arabidopsis

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-01-31 | GSE106972 | GEO

Mitotic chromosome condensation resets chromatin to maintain post-anaphase transcriptional homeostasis

Project description:Mitotic chromosome condensation resets chromatin to maintain post-anaphase transcriptional homeostasis

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Mitotic chromosome condensation resets chromatin to maintain post-anaphase transcriptional homeostasis (RNA-Seq II)

Project description:Mitotic chromosome condensation resets chromatin to maintain post-anaphase transcriptional homeostasis (RNA-Seq II)

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Mitotic chromosome condensation resets chromatin to maintain post-anaphase transcriptional homeostasis (RNA-Seq I)

Project description:Mitotic chromosome condensation resets chromatin to maintain post-anaphase transcriptional homeostasis (RNA-Seq I)

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Mitotic chromosome condensation resets chromatin to maintain post-anaphase transcriptional homeostasis (RNA-Seq III)

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ChIP-seq of DRD1, DMS3 and RDM1 in Arabidopsis

Project description:We performed FLAG ChIP-seq of transgenic lines expressing FLAG-tagged DRD1, DMS3 and RDM1, as well as Col0 as negative control.

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