Project description:This experiment aims on the identification of serine hydrolases from a complex thermophile community that live in a hot vent in Kamchatka Peninsula based on in vivo labelling with FP-alkyne directly in the hot spring and subsequent analysis using metagenomics/metaproteomics. To this end, sediment samples were collected and treated using the following three conditions. DMSO- treated control FP-alkyne labelled Samples for each condition were prepared in triplicate, resulting a total number of 6 samples per spring. Labelling was performed using 4 µM of the probe FP-alkyne and incubation for 2 h in the hot spring.
Project description:Here, we applied a microarray-based metagenomics technology termed GeoChip 5.0 to investigate spring microbial functional genes in mesocosm-simulated shallow lake ecosystems having been undergoing nutrient enrichment and warming for nine years.
Project description:We isolated an aerobic autotrophic bacterium from a hot spring on Kunashir Island and identified it as Thermus brevis strain PS18 cells were short straight nonmotile rods, 0.5x1.0-1-2 mkm. Strain PS18 grows autotrophically aerobically with thiosulfate (electron donor) and O₂ (acceptor). Autotrophy has been previously unknown in representatives of the Deinococcota phylum. Autotrophic growth of PS18 under aerobic and anaerobic conditions was characterized in growth experiments by proteomic analyses.
2026-02-19 | PXD070097 | Pride
Project description:16S rRNA METAGENOMICS OF NYS HOT SPRING SOLFATARIC MUD (OCT)