Project description:E. coli K12 strain W3110 was used in this study. Cells were grown anaerobically in defined medium at pH7 and 37°C in a stirred 3-liter bioreactor until the culture reached an OD (600nm) of 3. At that point the first sample was drawn and aeration was started subsequently at 1l/min. 0.5, 1, 2, 5, and 10 min after the onset of aeration additional samples were drawn.
Project description:Salmonella typhimurium 14028s Transposon library grown in M9 minimal medium (arabinose 0.4%), O/N at 37°C with aeration, compared to the initial library selected on Luria agar plates + kanamycin (50ug/ml), O/N at 37°C Keywords: Transposon tag analysis
Project description:The regulatory role of the Fis protein in fis and in the transcription of several gene regions during mid-exponential and late-stationary phase, and during different growth aeration regimes, has been investigated. Studies were done during those two growth phases and in aerated and non-aerated (microaerobic) conditions, to measure Fis enrichment and binding peaks in strategic gene regions by genome-wide microarray analysis ChIP-chip. This research investigation points to central roles for SPI-1, SPI-2, DNA gyrase and topoisomerase I, the elements of the stringent response, and the regulatory function of Fis-binding patterns, in setting and re-setting the activity of the fis gene and other involved promoters as a function of the growth conditions and aeration regimes experienced by Salmonella.
Project description:Our research describe the influence of aeration conditions in petri dishes for A. thaliana growth. We analyze the difference between plants grown in standard Petri dish (Non-aerated) and modified Petri dish that include aeration (Aerated). To characterize the differences between those conditions the gene expression analysis was performed. We also wanted to analyze the effect of using a micropore filter, so we designed another experiment with Aerated, Non-aerated and Micropore filter conditions.
Project description:Salmonella typhimurium 14028s Transposon library recovered from spleen after IP passage of 2x10^6 through BALB/c mouse, compared to the initial Transposon library grown in LB broth (kanamycin 50ug/ml), O/N at 37°C with aeration Keywords: Transposon tag analysis
Project description:The utilisation of microbes for the production of enzymes and pharmaceutical proteins is an important step towards a sustainable future. However, industrial fermentation processes require energy for agitation, aeration and cooling. This energy requirement increases with the increasing microbial cell mass. To address this technological challenge, we previously developed a so-called ‘breathing’ fermentation vessel that allows effective gas exchange. The present study aimed to explore the potential application of the breathing vessel for enzyme production by the Gram-positive bacterial cell factory Bacillus subtilis. In particular, we compared production of the secreted α-amylase AmyQ by the genome-reduced B. subtilis strain IIG-Bs-27-31 during parallel culturing in breathing vessels and shake flasks. Enzyme yields and the cellular and extracellular proteome compositions were compared.
Project description:Gene expression profiles of Bacillus subtilis strain AG174 were compared as a function of steady-state external pH during growth with aeration. Aerated overnight cultures were diluted 1:500 and incubated in 250 mL baffled flasks containing potassium-modified Luria-Bertani medium (LBK) buffered with 50 mM HOMOPIPES at pH 6.0, pH 7.0, and pH 9.0. Tubes were incubated at 37°C with aeration (260 rpm) to an optical density at 600 nm of 0.2. For each of the three pH conditions, RNA was isolated from five independent cultures.
Project description:RNA-seq study that inverstigated the transcriptomics landscape of Synechococcus sp. PCC 7002 under air (0.04%), optimal growth (4 & 8%) and growth-inhibiting high (30% CO2) aeration.