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Study gene expression specifically regulated by intrinsically active HOG1

ABSTRACT: Study gene expression specifically regulated by intrinsically active HOG1

PROVIDER: PRJNA420362 | ENA |

REPOSITORIES: ENA

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Study gene expression specifically regulated by intrinsically active HOG1

Project description:To monitor changes in the transcriptome following activation of Hog1 per se, we used a previously established inducible expression system of intrinsically active Hog1 proteins

2017-11-30 | GSE107491 | GEO

Dynamics of calcineurin-regulated gene expression in the absence of Hog1 in S. cerevisiae

Project description:Gene expression was quantified in crz1 hog1 delete S. cerevisae cells over a time course following calcineurin activation by CaCl2. Cells were pretreated with buffer or the calcineurin inhibitor FK506 to allow the identification of calcineurin-regulated genes. We show that calcineurin-dependent downregulation of G1/S genes is partly dependent on the osmostress-activated kinase Hog1.

2020-01-14 | GSE115014 | GEO

Hog1 ChIP Chip

Project description:Yeast Hog1-3HA ChIP-Chip in triplicate Keywords: ChIP-Chip

2006-05-26 | GSE4862 | GEO

Hog1 ChIP Chip

Project description:Yeast Hog1-3HA ChIP-Chip in triplicate Keywords: ChIP-Chip Yeast Hog1 ChIP-Chip was performed in MAP51 (3HA-Hog1) in triplicate using anti-HA (12CA5 monoclonal antibody)

2010-07-01 | E-GEOD-4862 | biostudies-arrayexpress

Transcription profiling of wild type and hog1 Candida albicans subjected to environmental stresses that are known to activate the Hog1 Stress-activated Protein Kinase

Project description:Based on studies in S. pombe (Chen et al., 2003), we predicted that conditions which activate C. albicans Hog1 would result in the induction of a common set of genes that are regulated by this SAPK (Smith et al., 2004). Hence in this study we compared the global transcriptional responses of wild-type and hog1 C. albicans cells to environmental stresses that are known to activate the Hog1 SAPK. We compared the homozygous hog1/hog1 null mutant (JC50) with the isogenic HOG1 reintegrant (hog1/hog1/HOG1: JC52) because this controlled for any secondary mutations that might have been introduced during the construction of the null mutant. We have shown that this reintegrant is indistinguishable from its parental wild-type strain RM1000 (HOG1/HOG1) with respect to their stress phenotypes (Smith et al., 2004) and their expression of stress genes (Supplementary Data). Three conditions were chosen for transcript profiling: osmotic stress imposed by 0.3 M NaCl, oxidative stress imposed by 5 mM H2O2, and heavy metal stress imposed by 0.5 mM CdSO4. Each of these treatments stimulates the phosphorylation and nuclear accumulation of this Hog1 SAPK within a 10-min time frame (Smith et al., 2004). Furthermore, significant differences in stress regulated gene expression are observed within this time scale (Enjalbert et al., 2003; Smith et al., 2004). Hence, we analyzed the C. albicans transcriptome after a 10-min exposure to each stress condition. Although some stress genes might be missed by analyzing a single time point, most C. albicans stress genes are induced within 10 min (Enjalbert et al., 2003). At least four independent biological replicates were analyzed for each condition

2007-07-05 | E-MEXP-1134 | biostudies-arrayexpress

Nuclear Localization of Hog1 MAPK is Not Necessary for Resistance to Hyperosmotic Stress

Project description:In budding yeast, this signaling pathway— the high-osmolarity glycerol (HOG) response —culminates in dual phosphorylation and nuclear translocation of the MAPK, Hog1 (ortholog of mammalian p38/SAPK). Induction of at least 50 genes requires nuclear Hog1, implying that transcriptional up-regulation is necessary to cope with hyperosmotic stress. Contrary to this expectation, we found that cells lacking the karyopherin (Nmd5) required for Hog1 nuclear import or in which Hog1 was permanently anchored at the plasma membrane(HOG1-CCAAX) (or both) withstood hyperosmotic challenge by three different solutes (1 M sorbitol, KCl or NaCl). In cells where activated Hog1 is excluded from the nucleus, there was little change in transcriptional program after exposure to hyperosmotic shock (comparable to hog1∆ cells), as judged by examining several diagnostic mRNAs and by global transcript measurements using microarrays. Systematic genetic analysis ruled out the need for any transcription factor known to be influenced by Hog1 (Hot1, Msn2, Msn4, Sko1 and Smp1). Keywords: Time course of stress response gene expression array

2008-06-01 | GSE8703 | GEO

Dynamics of calcineurin-regulated gene expression in the absence of Hog1 in S. cerevisiae

Project description:Dynamics of calcineurin-regulated gene expression in the absence of Hog1 in S. cerevisiae

| PRJNA473520 | ENA

Replication licensing regulated by an intrinsically disordered region of origin recognition complex

Project description:Replication licensing regulated by an intrinsically disordered region of origin recognition complex

| PRJNA1093011 | ENA

hog1 mutant 3 hour exposure to zymolyase

Project description:We did transcription profiling on the effect of hog1 deletion, gene involved in cell osmotic stress and cell wall stress response Keywords: cell wall stress response

2008-09-06 | GSE9931 | GEO

Thiol-Based Direct Threat Sensing by the Stress-Activated Protein Kinase Hog1

Project description:The stress-activated protein kinase Hog1 is best known for its role in osmotic stress but is also activated by a variety of mechanistically distinct environmental stressors including heat shock, ER stress, and arsenic. In the osmotic stress response, the signal is sensed upstream and relayed to Hog1 via a kinase cascade. Here we identify a novel mode of Hog1 function whereby Hog1 senses arsenic though direct physical interaction requiring three conserved cysteine residues located adjacent to the catalytic loop. These residues are essential for Hog1's role in protecting against arsenic, but completely dispensable for osmotic stress, and mediate Hog1's cellular localization upon arsenic exposure. Hog1 in turn regulates arsenic detoxification by phosphorylating the transcription factor Yap8, promoting its nuclear localization, and stimulating transcription of its only known targets, Arr2 and Arr3, which promote arsenic efflux. Arsenic binding was also observed with the related human kinases Erk1 and Erk2, suggesting that this may be a conserved aspect of this kinase family. These data provide a mechanistic basis for understanding how stress-activated kinases can sense individual threats and carry out highly specific adaptive responses.

2019-12-04 | PXD015325 | Pride

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