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Nuclear dynamics of the Set1C subunit Spp1 prepares meiotic recombination sites for break formation

ABSTRACT: Nuclear dynamics of the Set1C subunit Spp1 prepares meiotic recombination sites for break formation

PROVIDER: PRJNA422055 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6367810.fastq.gz	Fastqsanger.gz
	SRR6367820.fastq.gz	Fastqsanger.gz
	SRR6367830.fastq.gz	Fastqsanger.gz
	SRR6367840.fastq.gz	Fastqsanger.gz
	SRR6367850.fastq.gz	Fastqsanger.gz

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Similar Datasets

Nuclear dynamics of the Set1C subunit Spp1 prepares meiotic recombination sites for break formation.

Project description:Spp1 is the H3K4me3 reader subunit of the Set1 complex (COMPASS/Set1C) that contributes to the mechanism by which meiotic DNA break sites are mechanistically selected. We previously proposed a model in which Spp1 interacts with H3K4me3 and the chromosome axis protein Mer2 that leads to DSB formation. Here we show that spatial interactions of Spp1 and Mer2 occur independently of Set1C. Spp1 exhibits dynamic chromatin binding features during meiosis, with many de novo appearing and disappearing binding sites. Spp1 chromatin binding dynamics depends on its PHD finger and Mer2-interacting domain and on modifiable histone residues (H3R2/K4). Remarkably, association of Spp1 with Mer2 axial sites reduces the effective turnover rate and diffusion coefficient of Spp1 upon chromatin binding, compared with other Set1C subunits. Our results indicate that "chromosomal turnover rate" is a major molecular determinant of Spp1 function in the framework of meiotic chromatin structure that prepares recombination initiation sites for break formation.

| S-EPMC6168271 | biostudies-literature

Nuclear dynamics of the Set1C subunit Spp1 prepares meiotic recombination sites for break formation

Project description:This study investigates the nuclear localizations and dynamics of COMPASS proteins using ChIP-seq and competition ChIP.

2018-07-17 | GSE107967 | GEO

CXXC1 is not essential for normal DNA double-strand break formation and meiotic recombination in mouse

Project description:In most mammals, including mice and humans, meiotic recombination is determined by the meiosis specific histone methytransferase PRDM9, which binds to specific DNA sequences and trimethylates histone 3 at lysine-4 and lysine-36 at the adjacent nucleosomes. These actions ensure successful DNA double strand break formation and repair that occur on the proteinaceous structure forming the chromosome axis. The process of hotspot association with the axis after their activation by PRDM9 is poorly understood. Previously, we and others have identified CXXC1, an ortholog of S. cerevisiae Spp1 in mammals, as a PRDM9 interactor. In yeast, Spp1 is a histone methyl reader that links H3K4me3 sites with the recombination machinery, promoting DSB formation. Here, we investigated whether CXXC1 has a similar function in mouse meiosis. We created two Cxxc1 conditional knockout mouse to deplete CXXC1 generally in germ cells, and before the onset of meiosis. Surprisingly, male knockout mice were fertile, and the loss of CXXC1 in spermatocytes had no effect on hotspot trimethylation, double-strand break formation or repair. Our results demonstrate that CXXC1 is not an essential link between PRDM9-activated recombination hotspot sites and DSB machinery and that the hotspot recognition pathway in mouse is independent of CXXC1.

2018-08-29 | GSE116336 | GEO

Characterization of Spp1-TAP and Mer2-TAP interactomes

2018-01-30 | PXD007590 | Pride

Separable functions of the PHD finger protein Spp1 in the Set1 and the meiotic DSB forming complexes cooperate for meiotic DSB formation

Project description:Histone H3K4 methylation is a feature of meiotic recombination hotspots shared by many organisms including plants and mammals. Meiotic recombination is initiated by programmed double-strand break (DSB) formation that in budding yeast takes place in gene promoters and is promoted by histone H3K4 di/trimethylation. This histone modification is recognized by Spp1, a PHD-finger containing protein that belongs to the conserved histone H3K4 methyltransferase Set1 complex. During meiosis, Spp1 binds H3K4me3 and interacts with a DSB protein, Mer2, to promote DSB formation close to gene promoters. How Set1 complex- and Mer2- related functions of Spp1 are connected is not clear. Here, combining genome-wide localization analyses, biochemical approaches and the use of separation of function mutants, we show that Spp1 is present within two distinct complexes in meiotic cells, the Set1 and the Mer2 complexes. Disrupting the Spp1-Set1 interaction mildly decreases H3K4me3 levels and does not affect meiotic recombination initiation. Conversely, the Spp1-Mer2 interaction is required for normal meiotic recombination initiation, but dispensable for Set1 complex-mediated histone H3K4 methylation. Finally, we provide evidence that Spp1 preserves normal H3K4me3 levels independently of the Set1 complex. We propose a model where Spp1 works in three ways to promote recombination initiation: first by depositing histone H3K4 methylation (Set1 complex), next by “reading” and protecting histone H3K4 methylation, and finally by making the link with the chromosome axis (Mer2-Spp1 complex). This work deciphers the precise roles of Spp1 in meiotic recombination and opens perspectives to study its functions in other organisms where H3K4me3 is also present at recombination hotspots.

2017-11-30 | GSE102790 | GEO

Genome-wide nascent transcription patterns following deletion of COMPASS subunit SPP1

Project description:Genome-wide nascent transcription patterns following deletion of COMPASS subunit SPP1

| PRJNA552547 | ENA

Genome-wide levels of H3K4me3 following deletion of COMPASS subunit SPP1

Project description:Genome-wide levels of H3K4me3 following deletion of COMPASS subunit SPP1

| PRJNA552544 | ENA

Genome-wide levels of H3K4me3 following deletion of COMPASS subunit SPP1

Project description:We report a drastic genome-wide reduction of H3K4me3 levels following deletion of SPP1.

2019-07-04 | GSE133788 | GEO

Genome-wide nascent transcription patterns following deletion of COMPASS subunit SPP1

Project description:Despite the well-reported association between H3K4me3 and transcription, ablation of H3K4me3 by deletion of SPP1 has little effect on nascent transcription.

2019-07-04 | GSE133790 | GEO

Spp1, a member of Set1 complex, promotes meiotic DSB formation by tethering histone H3K4 methylation sites to chromosome axes

Project description:Meiotic DSB, catalyzed by the Spo11 transesterase protein and accessory DSB proteins, form in the nucleosome depleted regions (NDR) at promoters, preferentially those located on the chromosome loops that shape meiotic chromosomes, whereas the DSB proteins are located on chromosome axes at the basis of these loops. Mechanisms bridging these two chromosomal regions for DSB formation have remained elusive. Here we show that Spp1, a conserved member of the histone H3K4 methyltransferase Set1 complex, is required for normal levels of DSB formation and is associated with chromosome axes in the DSB-rich domains during meiosis. Moreover, Spp1 physically interacts with the Mer2 axis-associated DSB protein, and uses its PHD finger as a magnet to read H3K4 trimethylation close to promoters, tether these regions to chromosome axes and activate cleavage in the nearby promoter by the DSB proteins. We further show that in the absence of Spp1 or the Set1 complex, DSB are introduced at a few new sites, located in promoters of transcriptionally induced genes, suggesting another selection mechanism of preferred DSB sequences. This paper provides the molecular mechanism linking H3K4me3 to the DSB forming machinery, by the meiosis-specific specialization of Spp1 as an active member of the DSB complex and a reader of H3K4me3, and opens perspectives for the study of DSB formation at mammalian recombination hotspots that are also enriched in H3K4me3. ChIP-chip experiment in vegetative or meiotic diploid SK1 yeast cells - two biological replicates

2012-12-13 | E-GEOD-39900 | biostudies-arrayexpress

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