Acute treatment with kynurenic acid in inguinal primary adipocytes
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ABSTRACT:
PROVIDER: PRJNA422606 | ENA |
REPOSITORIES: ENA
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Project description:Tryptophan-kynurenine metabolism plays an important role in the pathogenesis of several psychiatric diseases but its physiological function in peripheral tissues remains unclear. Physical exercise training activates a biochemical pathway in skeletal muscle that protects from neuroinflammation and, as a byproduct, leads to kynurenic acid accumulation in the periphery. We therefore investigated the effects of kynurenic acid in murine white adipose tissue. We used microarrays to detail global programme of gene expression underlying Kynurenic acid mediated effects on inguinal primary adipocytes.
2017-12-16 | GSE108158 | GEO
Project description:Purpose: The goals of this study are to compare the transcription difference between purified dermal adipocytes and inguinal adipocytes Methods: Transcriptome of purified dermal adipocytes and inguinal adipocytes were generated by deep sequencing, using Illumina Hiseq 2500 v3 sequencing system.
2019-09-16 | GSE128719 | GEO
Project description:Transcriptome sequencing of purified dermal adipocytes and inguinal adipocytes
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Project description:Circadian Regulation of Murine Inguinal Adipocytes
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Project description:We derived a model that allows for doxycycline-inducible deletion of Zfp423 in mature adipocytes of adult mice (Adiponectin-rtTA; TRE-CRE; Zfp423 loxP/loxP). In these animals deletion of Zfp423 results in a spontaneous conversion of white adipocytes into beige-like adipocytes at room temperature. The goal of this expression analysis was to 1) determine the gene programs dependent on adipocyte Zfp423 in inguinal WAT, and 2) determine the similarity between the converted beige-like cells to normal beige adipose tissue that accumulates upon cold exppsure.
2016-02-10 | GSE74899 | GEO
Project description:Here we have employed chromatin immunoprecipitation combined with deep sequencing to map and compare PPARM-NM-3 binding in in vitro differentiated primary mouse adipocytes isolated from epididymal, inguinal, and brown adipose tissues. While these PPARM-NM-3 binding profiles are overall similar, there are clear depot-selective binding sites. Most PPARM-NM-3 binding sites previously mapped in 3T3-L1 adipocytes can also be detected in primary adipocytes, but there are a large number of PPARM-NM-3 binding sites that are specific to the primary cells, and these tend to be located in closed chromatin regions in 3T3-L1 adipocytes. The depot-selective binding of PPARM-NM-3 is associated with highly depot-specific gene expression. This indicates that PPARM-NM-3 plays a role in the induction of genes characteristic of different adipocyte lineages and that preadipocytes from different depots are differentially preprogrammed to permit PPARM-NM-3 lineage-specific recruitment even when differentiated in vitro. Examination of PPARM-NM-3 binding in in vitro differentiatied adipocytes isolated from three different adipose depots.
2012-10-11 | E-GEOD-41481 | biostudies-arrayexpress
Project description:Here we have employed chromatin immunoprecipitation combined with deep sequencing to map and compare PPARγ binding in in vitro differentiated primary mouse adipocytes isolated from epididymal, inguinal, and brown adipose tissues. While these PPARγ binding profiles are overall similar, there are clear depot-selective binding sites. Most PPARγ binding sites previously mapped in 3T3-L1 adipocytes can also be detected in primary adipocytes, but there are a large number of PPARγ binding sites that are specific to the primary cells, and these tend to be located in closed chromatin regions in 3T3-L1 adipocytes. The depot-selective binding of PPARγ is associated with highly depot-specific gene expression. This indicates that PPARγ plays a role in the induction of genes characteristic of different adipocyte lineages and that preadipocytes from different depots are differentially preprogrammed to permit PPARγ lineage-specific recruitment even when differentiated in vitro.
2012-10-11 | GSE41481 | GEO
Project description:We analyzed coding and noncoding transcript abundance in primary differentiating brite adipocytes derived from murine inguinal white adipose tissue, 24 hours in response to lncRNA Ctcflos knockdown at day 1 of differentiation
2021-03-19 | GSE169151 | GEO
Project description:The focus of this research is in the area of biological clocks. Importance: Metabolic dysregulation is the major preventable risk factor for leading causes of chronic disease-related deaths. More specifically, chronic obesity is correlated with adipocyte hypertrophy and hyperplasia, both of which may be circadianly regulated. All circadian clocks are cell-intrinsic, and circadian oscillators that are tissue-specific control metabolic homeostasis by fine-tuning nutrient utilization; adipose tissue responds to microenvironmental changes in a clock-dependent manner. The objective of this work is to understand how circadian rhythms affect adipocyte biology. To delineate the relationship between the cellular circadian system and adipocyte biology in the absence of organismal cues, circadian output was characterized by transcriptionally profiling in vitro differentiated adipocytes from inguinal adipose tissue over 3 circadian days with a 2-hour resolution via RNAseq. Goal: Determine what aspects of adipocyte biology and environmental stimuli can be influenced by time-of-day. Impact: Findings from this study will increase our understanding of clock-controlled energy metabolism and adipocyte dysfunction, advancing our understanding of the non-linear association between weight, energy expenditure and risk in chronic disease. Purpose: The goals of this study are to understand the molecular basis of the biological clock in mammals and the means through which it controls metabolism. PER2::LUC transgenic mice were used as a source of adipocyte stem cells for studies on the role of the clock in adipocyte biology. Methods: There are two biological replicates. 4 male mice aged 8-12 weeks under 12:12 light-dark cycle and standard chow were used per replicate. The inguinal stromal vascular fraction (SVF) was isolated post-mortem at ZT10*lights on, resting phase, 10am*. Isofluorine anesthesia and cervical dislocation were the method of sacrifice. Pre-adipocytes were isolated as in (Oeckl 2020, DOI: 10.1016/ j.xpro.2020.100118) with modifications and seeded onto three 35mM plastic bottom tissue culture plates (MatTek) in "base media" (DMEM F/12 + GlutaMAX (Gibco xxxx-018) with penicillin/streptomycin and 10% fetal bovine serum (Gibco: Ref#10437-028). Cells were passaged every 48 hours for a total of 3 passages with a split ratio of 1:2.8. In final passage, cells were seeded into 35mM plastic dishes (MatTek). After confluence, differentiation was induced with dexamethasone, rosiglitazone and IBMX, biotin, insulin, pantothenate ( "differentiation media"). After 4 days of differentiation, dex, rosi and IBMX were removed from the media ( "maintenance media"). After 4 days in culture, cells were synchronized with 50% FBS, 50% "base media" and 10uM forskolin for 2-2.5hours. Media was switched to "maintenance media" containing 0.01% bright d-luciferin (GoldBio #LUCK-100) in batches of 4 dishes, topped with glass microscopy slides and sealed with vacuum grease before loading into a Lumicycle housed in a 37C, 0%CO2 incubator. Circadian parameters including amplitude were determined using LumiCycle Analysis software (Actimetrics, v.2.44).
2025-04-22 | GSE202686 | GEO
Project description:We analyzed coding and noncoding transcript abundance in primary differentiating brite adipocytes derived from murine inguinal white adipose tissue, 24 hours or 72 hours in response to lncRNA Ctcflos knockdown at day 1 of differentiation
2021-03-19 | GSE169150 | GEO