Circadian Regulation of Murine Inguinal Adipocytes
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA836938 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR19160300_1.fastq.gz | Fastqsanger.gz | |||
| SRR19160300_2.fastq.gz | Fastqsanger.gz | |||
| SRR19160301.fastq.gz | Fastqsanger.gz | |||
| SRR19160301_1.fastq.gz | Fastqsanger.gz | |||
| SRR19160301_2.fastq.gz | Fastqsanger.gz |
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Project description:The focus of this research is in the area of biological clocks. Importance: Metabolic dysregulation is the major preventable risk factor for leading causes of chronic disease-related deaths. More specifically, chronic obesity is correlated with adipocyte hypertrophy and hyperplasia, both of which may be circadianly regulated. All circadian clocks are cell-intrinsic, and circadian oscillators that are tissue-specific control metabolic homeostasis by fine-tuning nutrient utilization; adipose tissue responds to microenvironmental changes in a clock-dependent manner. The objective of this work is to understand how circadian rhythms affect adipocyte biology. To delineate the relationship between the cellular circadian system and adipocyte biology in the absence of organismal cues, circadian output was characterized by transcriptionally profiling in vitro differentiated adipocytes from inguinal adipose tissue over 3 circadian days with a 2-hour resolution via RNAseq. Goal: Determine what aspects of adipocyte biology and environmental stimuli can be influenced by time-of-day. Impact: Findings from this study will increase our understanding of clock-controlled energy metabolism and adipocyte dysfunction, advancing our understanding of the non-linear association between weight, energy expenditure and risk in chronic disease. Purpose: The goals of this study are to understand the molecular basis of the biological clock in mammals and the means through which it controls metabolism. PER2::LUC transgenic mice were used as a source of adipocyte stem cells for studies on the role of the clock in adipocyte biology. Methods: There are two biological replicates. 4 male mice aged 8-12 weeks under 12:12 light-dark cycle and standard chow were used per replicate. The inguinal stromal vascular fraction (SVF) was isolated post-mortem at ZT10*lights on, resting phase, 10am*. Isofluorine anesthesia and cervical dislocation were the method of sacrifice. Pre-adipocytes were isolated as in (Oeckl 2020, DOI: 10.1016/ j.xpro.2020.100118) with modifications and seeded onto three 35mM plastic bottom tissue culture plates (MatTek) in "base media" (DMEM F/12 + GlutaMAX (Gibco xxxx-018) with penicillin/streptomycin and 10% fetal bovine serum (Gibco: Ref#10437-028). Cells were passaged every 48 hours for a total of 3 passages with a split ratio of 1:2.8. In final passage, cells were seeded into 35mM plastic dishes (MatTek). After confluence, differentiation was induced with dexamethasone, rosiglitazone and IBMX, biotin, insulin, pantothenate ( "differentiation media"). After 4 days of differentiation, dex, rosi and IBMX were removed from the media ( "maintenance media"). After 4 days in culture, cells were synchronized with 50% FBS, 50% "base media" and 10uM forskolin for 2-2.5hours. Media was switched to "maintenance media" containing 0.01% bright d-luciferin (GoldBio #LUCK-100) in batches of 4 dishes, topped with glass microscopy slides and sealed with vacuum grease before loading into a Lumicycle housed in a 37C, 0%CO2 incubator. Circadian parameters including amplitude were determined using LumiCycle Analysis software (Actimetrics, v.2.44).
2025-04-22 | GSE202686 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE25323: Biological Aging and Circadian Mechanisms in Murine Brown Adipose Tissue, Inguinal White Adipose Tissue, and Liver (Nov 2009 dataset) GSE25324: Biological Aging and Circadian Mechanisms in Murine Brown Adipose Tissue, Inguinal White Adipose Tissue, and Liver (Jan 2010 dataset) Refer to individual Series
2012-03-15 | E-GEOD-25325 | biostudies-arrayexpress
Project description:Purpose: The goals of this study are to compare the transcription difference between purified dermal adipocytes and inguinal adipocytes Methods: Transcriptome of purified dermal adipocytes and inguinal adipocytes were generated by deep sequencing, using Illumina Hiseq 2500 v3 sequencing system.
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Project description:Peripheral circadian clocks regulate many aspects of physiology. In this study we deleted the core circadian clock component Bmal1 specifically in mouse adipocytes in order to study the role of the adipocyte clock in energy homeostasis and body weight. We used microarrays to indentify changes in gene expression in the adipose tissue of mice lacking a functional adipocyte circadian clock and identified a small number of up- and down- regulated genes. Adipose tissues were isolated from inguinal adipose fat pads at four different times of the diurnal cycle under constant darkness (circadian time, CT) for RNA extraction and hybridization on Affymetrix microarrays. Adipocyte-specific Bmal1 KO (Ad-Bmal1-/-) and control mice were used for isolation of tissues at CT0, CT6, CT12, CT18 where CT0 the beginning of the subjective day.
2012-10-21 | E-GEOD-35026 | biostudies-arrayexpress
Project description:Transcriptome sequencing of purified dermal adipocytes and inguinal adipocytes
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Project description:Tryptophan-kynurenine metabolism plays an important role in the pathogenesis of several psychiatric diseases but its physiological function in peripheral tissues remains unclear. Physical exercise training activates a biochemical pathway in skeletal muscle that protects from neuroinflammation and, as a byproduct, leads to kynurenic acid accumulation in the periphery. We therefore investigated the effects of kynurenic acid in murine white adipose tissue. We used microarrays to detail global programme of gene expression underlying Kynurenic acid mediated effects on inguinal primary adipocytes.
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Project description:The circadian clock is an endogenous oscillator that drives daily rhythms in physiology, behavior and gene expression. The underlying mechanisms of circadian timekeeping are cell-autonomous and involve interconnecting transcription-translation feedback loops. The hippocampus plays an important role in spatial memory and the conversion of short-term to long-term memory. Several studies have reported the presence of a peripheral oscillator in the hippocampus, and have highlighted the importance of circadian regulation in memory formation. In this study we performed global quantitative proteome and phosphoproteome analyses of the murine hippocampus across the circadian cycle, applying spiked-in labeled reference and high accuracy mass spectrometry.
2017-04-11 | PXD005668 | Pride
Project description:We derived a model that allows for doxycycline-inducible deletion of Zfp423 in mature adipocytes of adult mice (Adiponectin-rtTA; TRE-CRE; Zfp423 loxP/loxP). In these animals deletion of Zfp423 results in a spontaneous conversion of white adipocytes into beige-like adipocytes at room temperature. The goal of this expression analysis was to 1) determine the gene programs dependent on adipocyte Zfp423 in inguinal WAT, and 2) determine the similarity between the converted beige-like cells to normal beige adipose tissue that accumulates upon cold exppsure.
2016-02-10 | GSE74899 | GEO
Project description:Acute treatment with kynurenic acid in inguinal primary adipocytes
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Project description:Peripheral circadian clocks regulate many aspects of physiology. In this study we deleted the core circadian clock component Bmal1 specifically in mouse adipocytes in order to study the role of the adipocyte clock in energy homeostasis and body weight. We used microarrays to indentify changes in gene expression in the adipose tissue of mice lacking a functional adipocyte circadian clock and identified a small number of up- and down- regulated genes.
2012-10-21 | GSE35026 | GEO