Project description:We analyzed the changes in the brain tissue of Apis mellifera ligustica at the molecular level by sequencing after using fluvalinate. We found that the differentially expressed miRNAs (DEM) may be involved in hippocampal cell apoptosis and damage to memory functions. This result may be related to behaviors observed after the administration of this medication, such as a lack of homing at night and behavioral disturbances. Overall, our results provide new information about the molecular mechanisms and pathways of fluvalinate action in the brain tissue of Apis mellifera ligustica.
Project description:Apis mellifera syriaca is the native honeybee subspecies of Jordan and much of the Middle East. It expresses behavioral adaptations to a regional climate with very high temperatures, nectar dearth in summer, attacks of the Oriental wasp Vespa orientalis and in most cases it is resistant to varroa mites. The Thorax control sample of A. m. syriaca in this experiment was originally collected and stored since 2001 from Wadi Ben Hammad a remote valley in the southern region of Jordan. Using morphometric and Mitochondrial DNA markers it was proved that bees from this area had show higher similarity than other samples collected from the Middle East as represented by reference samples collected in 1952 by Brother Adam. The samples L1-L5 are collected from the National Center for Agricultural Research and Extension breading apiary which was originally established for the conservation of Apis mellifera syriaca. Goal was to use the genetic information in the breeding for varroa resistant bees and to determine the successfulness of this conservation program. Project funded by USAID-MERC grant number: TA-MOU-09-M29-075.
Project description:Double stranded RNAs are used to induce gene silencing in functional studies. In Apis mellifera green fluorescent protein (GFP) dsRNA (dsGFP) has been used as an exogenous control as its sequence has no homology in honeybee genome. However, some undesirable effects are observed after dsGFP treatment. A microarray approach comparing gene expression differences between untreated and dsGFP treated groups, containing honeybees workers in two different developmental stages, pre-pupae and light-brown eyed pupae, were used to test the reliability of dsGFP as a control for RNAi experiments. According to these microarrays results dsGFP can be used as a control in RNAi assays as long as the affected genes are taken into account in the analysis.
Project description:The microsporidia Nosema ceranae are intracellular parasites that proliferate in the midgut epithelial cells of honey bees (Apis mellifera). To analyze the pathological effects of those microsporidia, we orally infected honey bee workers 7 days after their emergence. Bees were flash frozen 15 days after the infection. Then, the effects on the gut ventriculi were analyzed and compared to non-infected (control) bees.
Project description:Double stranded RNAs are used to induce gene silencing in functional studies. In Apis mellifera green fluorescent protein (GFP) dsRNA (dsGFP) has been used as an exogenous control as its sequence has no homology in honeybee genome. However, some undesirable effects are observed after dsGFP treatment. A microarray approach comparing gene expression differences between untreated and dsGFP treated groups, containing honeybees workers in two different developmental stages, pre-pupae and light-brown eyed pupae, were used to test the reliability of dsGFP as a control for RNAi experiments. According to these microarrays results dsGFP can be used as a control in RNAi assays as long as the affected genes are taken into account in the analysis. Analysis used loop-design to compare dsGFP treated and noon-treated workers in pre-pupae and light-brown-eyed pupae stages.
