Project description:Dogs are highly valued companions and work animals that are susceptible to many life-threatening conditions like canine leishmaniosis (CanL). Plasma-derived extracellular vesicles (EVs), exploited extensively in biomarker discovery, constitute a mostly untapped resource in veterinary sciences. Thus, the definition of proteins associated with plasma EVs recovered from healthy and diseased dogs with a relevant pathogen would be important for biomarker development. For this, we recovered using size-exclusion chromatography (SEC), EVs from 19 healthy and 20 CanL dogs’ plasma and performed proteomic analysis by LC-MS/MS to define their core proteomic composition and search for CanL-associated alterations. EVs-specific markers were identified in all preparations and also non-EVs proteins. Some EVs markers like CD82 were specific to the healthy animals, while others like the Integrin beta 3 were identified in most samples. The EVs-enriched preparations allowed the identification of 529 canine proteins that were identified in both groups, while 465 and 154 were only identified in healthy or CanL samples, respectively. A GO enrichment analysis revealed few CanL-specific terms. Leishmania spp. protein identifications were also found alt-hough with only 1 unique peptide. Ultimately, CanL-associated proteins of interest were identified and a core proteome was revealed that will be available for intra- and inter-species comparisons.

2023-05-10 | PXD039610 | Pride

Ancylostoma caninum strain:Baltimore

Project description:Ancylostoma caninum Genome Sequencing

| PRJNA72585 | ENA

Ancylostoma caninum

Project description:Ancylostoma caninum

| PRJNA12840 | ENA

Ancylostoma ceylanicum

Project description:Ancylostoma ceylanicum

| PRJNA12860 | ENA

Ancylostoma duodenale

Project description:Ancylostoma duodenale