Dataset Information

Characterization and proteomic analysis of plasma EVs recovered from healthy and diseased dogs with Canine Leishmaniosis

ABSTRACT: Dogs are highly valued companions and work animals that are susceptible to many life-threatening conditions like canine leishmaniosis (CanL). Plasma-derived extracellular vesicles (EVs), exploited extensively in biomarker discovery, constitute a mostly untapped resource in veterinary sciences. Thus, the definition of proteins associated with plasma EVs recovered from healthy and diseased dogs with a relevant pathogen would be important for biomarker development. For this, we recovered using size-exclusion chromatography (SEC), EVs from 19 healthy and 20 CanL dogs’ plasma and performed proteomic analysis by LC-MS/MS to define their core proteomic composition and search for CanL-associated alterations. EVs-specific markers were identified in all preparations and also non-EVs proteins. Some EVs markers like CD82 were specific to the healthy animals, while others like the Integrin beta 3 were identified in most samples. The EVs-enriched preparations allowed the identification of 529 canine proteins that were identified in both groups, while 465 and 154 were only identified in healthy or CanL samples, respectively. A GO enrichment analysis revealed few CanL-specific terms. Leishmania spp. protein identifications were also found alt-hough with only 1 unique peptide. Ultimately, CanL-associated proteins of interest were identified and a core proteome was revealed that will be available for intra- and inter-species comparisons.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Leishmania Infantum Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Blood Plasma

DISEASE(S): Leishmaniasis

SUBMITTER: Nuno Santarém

LAB HEAD: Anabela Cordeiro da Silva

PROVIDER: PXD039610 | Pride | 2023-05-10

REPOSITORIES: Pride

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Publications

Characterization and Proteomic Analysis of Plasma EVs Recovered from Healthy and Diseased Dogs with Canine Leishmaniosis.

Esteves Sofia S Lima Clara C Costa Inês I Osório Hugo H Fernandez-Becerra Carmen C Santarém Nuno N Cordeiro-da-Silva Anabela A

International journal of molecular sciences 20230313 6

Dogs are highly valued companions and work animals that are susceptible to many life-threatening conditions such as canine leishmaniosis (CanL). Plasma-derived extracellular vesicles (EVs), exploited extensively in biomarker discovery, constitute a mostly untapped resource in veterinary sciences. Thus, the definition of proteins associated with plasma EVs recovered from healthy and diseased dogs with a relevant pathogen would be important for biomarker development. For this, we recovered, using ...[more]

PMID: 36982564

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Dataset Information

Characterization and proteomic analysis of plasma EVs recovered from healthy and diseased dogs with Canine Leishmaniosis

Publications

Characterization and Proteomic Analysis of Plasma EVs Recovered from Healthy and Diseased Dogs with Canine Leishmaniosis.

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