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Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline [RRBS]

ABSTRACT: Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline [RRBS]

PROVIDER: PRJNA431944 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6517640.fastq.gz	Fastqsanger.gz
	SRR6517641.fastq.gz	Fastqsanger.gz
	SRR6517642.fastq.gz	Fastqsanger.gz
	SRR6517643.fastq.gz	Fastqsanger.gz
	SRR6517644.fastq.gz	Fastqsanger.gz

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Similar Datasets

Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline [RRBS]

Project description:Maternal Vitamin C is required in vivo for proper DNA demethylation and development of fetal germ cells in a mouse model of Vitamin C deficiency. Withdrawal of Vitamin C from the maternal diet does not affect overall embryonic development but leads to defects in the fetal germline, which persist well after Vitamin C re-supply during late gestation. The transcriptome of germ cells from Vitamin C-deficient embryos is remarkably similar to that of embryos carrying a mutation in Tet1, which is responsible for DNA demethylation and activation of regulators of meiosis. In agreement with these results, Vitamin C deficiency leads to an aberrant DNA methylation profile that includes incomplete demethylation of key regulators of meiosis and transposable elements. These findings reveal that deficiency in Vitamin C during gestation recapitulates a mutation in Tet1 and disrupts germline reprogramming and development. Our work further indicate that the embryonic germline is sensitive to perturbations of the maternal diet, providing a potential intergenerational mechanism for adjusting fecundity to environmental quality.

2019-07-01 | GSE109746 | GEO

Maternal Vitamin C regulates DNA demethylation and devlopment of the mouse embryonic germline [Cut&Run]

2019-07-01 | GSE125496 | GEO

Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline [RNA-Seq]

2019-07-01 | GSE109745 | GEO

Maternal Vitamin C regulates DNA demethylation and devlopment of the mouse embryonic germline [soma RNA-seq]

2019-07-01 | GSE125495 | GEO

Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline

Project description:Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline

| PRJNA431912 | ENA

Role of Tet3 and DNA replication in zygotic demethylation of both paternal and maternal genomes [RRBS]

Project description:With the exception of imprinted genes and certain repeats, DNA methylation is globally erased during pre-implantation development. Recent studies have suggested that Tet3-mediated oxidation of 5-methylcytosine (5mC) and DNA replication-dependent dilution both contribute to global paternal DNA demethylation, but demethylation of the maternal genome occurs via replication. Here we present genome-scale DNA methylation maps for both the paternal and maternal genomes of Tet3-depleted and/or DNA replication-inhibited zygotes. In both genomes, we found that inhibition of DNA replication blocks DNA demethylation independently from Tet3 function, and that Tet3 facilitates DNA demethylation by coupling with DNA replication. For both, our data indicate that replication-dependent dilution is the major contributor to demethylation, but Tet3 plays an important role, particularly at certain loci. Our study therefore both defines the respective functions of Tet3 and DNA replication in paternal DNA demethylation and reveals an unexpected contribution of Tet3 to demethylation of the maternal genome. In this data set, we include RRBS data of manually isolated paternal and maternal pronuclei from both WT and Tet3 CKO zygotes with or without aphidicolin treatment

2014-10-21 | E-GEOD-61330 | biostudies-arrayexpress

Maternal Vitamin C regulates DNA demethylation and devlopment of the mouse embryonic germline [Cut&Run]

Project description:Maternal Vitamin C regulates DNA demethylation and devlopment of the mouse embryonic germline [Cut&Run]

| PRJNA516596 | ENA

Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline [RNA-Seq]

Project description:Maternal Vitamin C regulates DNA demethylation and development of the mouse embryonic germline [RNA-Seq]

| PRJNA431945 | ENA

Maternal Vitamin C regulates DNA demethylation and devlopment of the mouse embryonic germline [soma RNA-seq]

Project description:Maternal Vitamin C regulates DNA demethylation and devlopment of the mouse embryonic germline [soma RNA-seq]

| PRJNA516595 | ENA

Vitamin C Induces Specific Demethylation of H3K9me2 in Mouse Embryonic Stem Cells via Kdm3a/b

Project description:Histone methylation patterns regulate gene expression and are highly dynamic during development. The erasure of histone methylation is carried out by histone demethylase enzymes. We had previously shown that vitamin C enhances the activity of Tet enzymes in embryonic stem (ES) cells, leading to DNA demethylation and activation of germline genes. We report here that vitamin C induces a remarkably specific demethylation of histone H3 lysine 9 dimethylation (H3K9me2) in ES cells. Vitamin C treatment reduces global levels of H3K9me2, but not other histone methylation marks analyzed, as measured by Western blot, immunofluorescence and mass spectrometry. Vitamin C leads to widespread loss of H3K9me2 at large chromosomal domains as well as gene promoters and repeat elements. Vitamin C-induced loss of H3K9me2 occurs rapidly within 24 hours and is reversible. Importantly, we found that the histone demethylases Kdm3a and Kdm3b are required for vitamin C-induced demethylation of H3K9me2. Moreover, we show that vitamin C-induced Kdm3a/b-mediated H3K9me2 demethylation and Tet-mediated DNA demethylation are independent processes. Lastly, we document Kdm3a/b are partially required for the up-regulation of germline genes by vitamin C. These results reveal a specific role for vitamin C in histone demethylation in ES cells, and document that DNA methylation and H3K9me2 cooperate to silence germline genes in pluripotent cells.

2017-06-30 | GSE84009 | GEO

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