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Profiling of alternative transcription start sites and alternative polyadenylation sites in Xenopus tropicalis

ABSTRACT: Profiling of alternative transcription start sites and alternative polyadenylation sites in Xenopus tropicalis

PROVIDER: PRJNA432702 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6666800.fastq.gz	Fastqsanger.gz
	SRR6666810.fastq.gz	Fastqsanger.gz
	SRR6666820.fastq.gz	Fastqsanger.gz
	SRR6666801.fastq.gz	Fastqsanger.gz
	SRR6666811.fastq.gz	Fastqsanger.gz

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Profiling of alternative transcription start sites and alternative polyadenylation sites in Xenopus tropicalis

Project description:We have developed both WTSS-seq (whole transcriptome start site sequencing) and WTTS-seq (whole transcriptome termini site sequencing) methods to capture either 5’- or 3’-ends of transcripts. HATT-seq (head and tail tag sequencing) is still under development, which can be used to capture both 5’- and 3’ ends of each transcript simultaneously. Iso-seq was used to produce full-length transcripts, which can be used to validate both alternative transcription start sites and alternative polyadenylation sites. CAGE-seq was used to confirm alternative transcription start sites only.

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Project description:Usage of Alternative Polyadenylation Sites Differs Dramatically Between Male and Female Xenopus tropicalis.