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Gene expression signatures before or after transient middle cerebral artery oclussion (tMCAO) in ZEB1 transgenic and wild type mice

ABSTRACT: Gene expression signatures before or after transient middle cerebral artery oclussion (tMCAO) in ZEB1 transgenic and wild type mice

PROVIDER: PRJNA432941 | ENA |

REPOSITORIES: ENA

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Gene expression signatures before or after transient middle cerebral artery oclussion (tMCAO) in ZEB1 transgenic and wild type mice

Project description:To further development of the role of ZEB1 in microglia, we have employed whole mRNA microarray expression profiling as a discovery platform to identify genes. Mouse microglia from wild type(wt), ZEB1-overexpression(ZEB1-tg) and ZEB1 knock down(ZEB1-kd) mice were isolated before and after tMCAO. The result showed after tMCAO, the wild type, ZEB1-overexpression and ZEB1 knock down microglia displayed a different gene profile, this suggested microglia ZEB1 might play an important role after ischemic stroke.

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Project description:This data set shows dramatic changes in gene expression in microglia isolated from C57Bl6/J mice subjected to transient middle cerebral artery occlusion, as compared to those subjected to sham surgery. Mice deficient in Mincle (Clec4e-/-) showed significantly improved injury outcomes 3 and 7 days after transient middle cerebral artery occlusion. However, when comparing changes in gene expression in microglia 24 hours after blood reperfusion, there were no differences between wild-type and Clec4e-/- mice, indicating that Mincle does not participate in early microglial activation. Wild type and Mincle knock-out (Clec4e-/-) mice. After 1 h of transient middle cerebral artery occlusion (tMCAO) and 24 h of reperfusion, mice were perfused with PBS, their brains dissected, and 2 ipsilesional hemispheres (with cerebellum and brainstem removed) pooled for microglia isolation. For sham-operated animals, the whole forebrain was used and brains were not pooled. After myelin separation by Percoll gradient centrifugation, around 80,000 CD45intermediate, CD11b+ microglial cells were sorted from each sample. Sham samples n=3, tMCAO samples n=5.

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| PRJNA767272 | ENA

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