Helice tientsinensis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA433035 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR7130655_1.fastq.gz | Fastqsanger.gz | |||
| SRR7130655_2.fastq.gz | Fastqsanger.gz | |||
| SRR7130656_1.fastq.gz | Fastqsanger.gz | |||
| SRR7130656_2.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 4 of 4 |
Similar Datasets
Project description:Helice tientsinensis Transcriptome or Gene expression
| PRJNA432830 | ENA
Project description:Helice tientsinensis Transcriptome or Gene expression
| PRJNA464178 | ENA
Project description:Helice tientsinensis Transcriptome or Gene expression
| PRJNA464181 | ENA
Project description:Helice tientsinensis Transcriptome or Gene expression
| PRJNA464176 | ENA
Project description:Chitinase is a kind of glycoside hydrolase which is widely distributed in nature and encoded by multiple genes to catalyze the decomposition of chitin, which plays an important role in the molting and pathogen defense of crustaceans. However, the research on chitinase in crustaceans is mainly focused on a few species with economic value. In this study, full-length cDNA sequences of the HtCHT1, HtCHT3 and HtCHT4 genes were cloned from the mudflat crab Helice tientsinensis by RACE, and the sequences were analyzed. The results showed that the full-length 2,229 bp of HtCHT1 gene encoded 627 amino acids, while the full-length 2,191 bp of HtCHT3 gene produced 489 amino acids, and the full-length 3,312 bp of HtCHT4 gene encoded 664 amino acids. Bioinformatics analysis showed that all the obtained chitinase proteins had the glycosyl hydrolase family 18 (GH18) catalytic domain and chitin-binding domain (ChtBD2), furthermore, HtCHT1 and HtCHT4 proteins had signal peptide domains at N-terminal. Phylogenetic analysis showed that different types of chitinase were clustered, and HtCHTs were closely related to chitinases in the Eriocheir sinensis. Expression profile analysis showed that the HtCHT1, HtCHT3 and HtCHT4 were significantly expressed in hepatopancreas. Furthermore, the expression of three genes was significantly up-regulated in hepatopancreas after the Vibrio parahaemolyticus challenge. These results suggested that HtCHT1, HtCHT3 and HtCHT4 were belonged to the CHITINASE gene family in H. tientsinensis and were potentially involved in the antibacterial immune response. This study provides essential information for further research of chitinase in H. tientsinensis and even crustaceans.
| S-EPMC10022498 | biostudies-literature
Project description:In this study, the complete mitochondrial genome (mitogenome) of Helice sheni was amplified and analyzed. The mitogenome is 16,062 bp in length, encoding the standard set of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and one control region. The nucleotide frequency of the mitogenome is as follows: A, 34.81%; C, 23.24%; G, 12.68%; and T, 29.25%. Eight overlapping areas and 22 intergenic spacers were found in the complete mitogenome. The typical initiation codon (ATT) and stop codon (TAA) were observed in eight genes, respectively. The phylogenetic tree indicates that Helicana wuana is closely related to H. sheni.
| S-EPMC7800991 | biostudies-literature
Project description:Ngan Kee Ng, Tohru Naruse, and Hsi-Te Shih (2018) Helice epicure, a new species of varunid crab, is described from the Ryukyu Islands, Japan. The new species in the genus Helice De Haan, 1833 can be distinguished from its congeners by morphological differences in the chelae and suborbital crenulation. The morphological differences between the new species and a sister clade (the H. latimera complex, including H. latimera Parisi, 1918, H. tientsinensis Rathbun, 1931 and H. formosensis Rathbun, 1931) are supported by mitochondrial cytochrome oxidase subunit I (COI) divergences. However, the phylogenetic relationship within the H. latimera complex is unresolved for the time being.
| S-EPMC6517753 | biostudies-literature
Project description:Intestinal microbiota plays key roles in maintaining the health and homeostasis of the host. However, information about whether the formation of intestinal microbiota of wild aquatic animals is associated with habitat microbes is not fully understood. Here, intestine samples were collected from two wild crab species and sediment samples were collected from the habitat environment. The total DNA of each sample was extracted, and the V3-V4 regions of 16S rRNA were sequenced using the MiSeq platform. The purpose of this study was to investigate the composition and diversity of intestinal microbiota and habitat microbes, and bacterial community relationships between wild crab intestine and habitat sediment. In the present study, the composition and diversity of intestinal microbiota of the two crab species were different from the habitat microbes. In contrast, a similar composition and diversity of the intestinal microbiota were observed between two crab species. Moreover, the bacterial community relationships between crab intestine and habitat sediment were associated with intestinal regions. Further network analysis revealed that the network structure of the intestinal microbiota was not only associated with intestinal regions, but also with the crab species. Additionally, although the compositions of bacterial functions were similar between crab intestine and sediment, no significant correlation in bacterial functions was observed between crab intestine and sediment. The findings of the present study would contribute to understanding the relationship between intestinal microbiota of wild aquatic animal and habitat microbes, and providing new insights into the intestinal microbiota of wild aquatic animals.
| S-EPMC8551266 | biostudies-literature