Helice tientsinensis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA464178 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Helice tientsinensis Transcriptome or Gene expression
| PRJNA432830 | ENA
Project description:Helice tientsinensis Transcriptome or Gene expression
| PRJNA464181 | ENA
Project description:Helice tientsinensis Transcriptome or Gene expression
| PRJNA464176 | ENA
Project description:Chitinase is a kind of glycoside hydrolase which is widely distributed in nature and encoded by multiple genes to catalyze the decomposition of chitin, which plays an important role in the molting and pathogen defense of crustaceans. However, the research on chitinase in crustaceans is mainly focused on a few species with economic value. In this study, full-length cDNA sequences of the HtCHT1, HtCHT3 and HtCHT4 genes were cloned from the mudflat crab Helice tientsinensis by RACE, and the sequences were analyzed. The results showed that the full-length 2,229 bp of HtCHT1 gene encoded 627 amino acids, while the full-length 2,191 bp of HtCHT3 gene produced 489 amino acids, and the full-length 3,312 bp of HtCHT4 gene encoded 664 amino acids. Bioinformatics analysis showed that all the obtained chitinase proteins had the glycosyl hydrolase family 18 (GH18) catalytic domain and chitin-binding domain (ChtBD2), furthermore, HtCHT1 and HtCHT4 proteins had signal peptide domains at N-terminal. Phylogenetic analysis showed that different types of chitinase were clustered, and HtCHTs were closely related to chitinases in the Eriocheir sinensis. Expression profile analysis showed that the HtCHT1, HtCHT3 and HtCHT4 were significantly expressed in hepatopancreas. Furthermore, the expression of three genes was significantly up-regulated in hepatopancreas after the Vibrio parahaemolyticus challenge. These results suggested that HtCHT1, HtCHT3 and HtCHT4 were belonged to the CHITINASE gene family in H. tientsinensis and were potentially involved in the antibacterial immune response. This study provides essential information for further research of chitinase in H. tientsinensis and even crustaceans.
| S-EPMC10022498 | biostudies-literature
Project description:Interventions: A group:Before using cetuximab;B group:After progression of disease:no
Primary outcome(s): KRAS gene sequence;NRAS gene sequence;BRAF gene sequence;PIK3CA gene sequence;EGFR gene sequence;EGFR gene copy numbers;EGFR expression;Her2 expression;c-Met expression;PTEN expression
Study Design: historical control
| 2671639 | ecrin-mdr-crc
Project description:In this study, the complete mitochondrial genome (mitogenome) of Helice sheni was amplified and analyzed. The mitogenome is 16,062 bp in length, encoding the standard set of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and one control region. The nucleotide frequency of the mitogenome is as follows: A, 34.81%; C, 23.24%; G, 12.68%; and T, 29.25%. Eight overlapping areas and 22 intergenic spacers were found in the complete mitogenome. The typical initiation codon (ATT) and stop codon (TAA) were observed in eight genes, respectively. The phylogenetic tree indicates that Helicana wuana is closely related to H. sheni.
| S-EPMC7800991 | biostudies-literature
Project description:Ngan Kee Ng, Tohru Naruse, and Hsi-Te Shih (2018) Helice epicure, a new species of varunid crab, is described from the Ryukyu Islands, Japan. The new species in the genus Helice De Haan, 1833 can be distinguished from its congeners by morphological differences in the chelae and suborbital crenulation. The morphological differences between the new species and a sister clade (the H. latimera complex, including H. latimera Parisi, 1918, H. tientsinensis Rathbun, 1931 and H. formosensis Rathbun, 1931) are supported by mitochondrial cytochrome oxidase subunit I (COI) divergences. However, the phylogenetic relationship within the H. latimera complex is unresolved for the time being.
| S-EPMC6517753 | biostudies-literature