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Spatiotemporal pattern of the transcriptional regulatory landscape of the mouse ventricle after myocardial infarction

ABSTRACT: Spatiotemporal pattern of the transcriptional regulatory landscape of the mouse ventricle after myocardial infarction

PROVIDER: PRJNA433175 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6676810.fastq.gz	Fastqsanger.gz
	SRR6676820.fastq.gz	Fastqsanger.gz
	SRR6676830.fastq.gz	Fastqsanger.gz
	SRR6676811.fastq.gz	Fastqsanger.gz
	SRR6676821.fastq.gz	Fastqsanger.gz

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Transcription profiling of models of cardiac remodelling: myocardial infarction or transverse aortic constriction in left ventricle free wall and septum

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2013-11-15 | E-GEOD-47495 | biostudies-arrayexpress

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Project description:Despite a substantial progress in diagnosis and therapy, acute myocardial infarction (MI) is a major cause of mortality in the general population. A novel insight into the pathophysiology of myocardial infarction obtained by studying gene expression should help to discover novel biomarkers of MI and to suggest novel strategies of therapy. The aim of our study was to establish gene expression patterns in leukocytes from acute myocardial infarction patients. ST-segment elevation myocardial infarction alters expression of several groups of genes. On admission, several genes and pathways that could be directly or indirectly linked with lipid/glucose metabolism, platelet function and atherosclerotic plaque stability were affected (signaling of PPAR, IL-10, IL-6). Analysis at discharge highlighted specific immune response (upregulation of immunoglobulins). Highly significant and substantial upregulation of SOCS3 and FAM20 genes expression in the first 4-6 days of myocardial infarction in all patients is the most robust observation of our work Twenty-eight patients with ST-segment elevation myocardial infarction (STEMI) were included. The blood was collected on the 1st day of myocardial infarction, after 4-6 days, and after 6 months. Control group comprised 14 patients with stable coronary artery disease (CAD), without history of myocardial infarction. Gene expression analysis was performed with Affymetrix GeneChipM-BM-. Human Gene 1.0 ST microarrays and GCS3000 TG system.

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Project description:Purpose: rHCI hydrogel injection improves cardiac function within 2 days of treatment in a mouse model of myocardial infarction. The goal of this study was to determine the differences in gene expression with rHCI hydrogel injection post-myocardial infarction. Methods: Total RNA was isolated from left ventricle tissue of samples and mRNA stranded libraries were prepared and sequenced in Illumina NovaSeq 6000 with a depth of 50 million reads per sample. Differential expression analysis of aligned reads was done to compared gene expression between PBS vs. rHCI, PBS vs. sham, and rHCI vs. sham comparisons. Results: Differentially expressed genes between sham and PBS as well as rHCI and PBS showed known gene signatures of myocardial infarction. Seven differentially expressed genes were detected with rHCI hydrogel injection compared to PBS. Conclusions: The target gene ERDR1 that is downregulated with rHCI injection led to discovery of changes in cardiomyocyte apoptosis and reduction of oxidative stress adducts with rHCI injection post-myocardial infarction.

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myocardial infarction in Reg3beta knock-out mice

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2015-06-29 | E-MTAB-2561 | biostudies-arrayexpress

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