DNA:cytoplasm ratio defines an upper limit to cell size [array]
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PROVIDER: PRJNA435760 | ENA |
REPOSITORIES: ENA
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Project description:DNA:cytoplasm ratio defines an upper limit to cell size
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Project description:DNA:cytoplasm ratio defines an upper limit to cell size [RNA-seq]
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Project description:Cell size varies greatly between cell types, yet within a specific cell type and growth condition, cell size is narrowly distributed. Why maintenance of a cell-type specific cell size is important is not understood. Here we show that growing beyond a certain size has wide-ranging effects on cell physiology. Large cells are defective in gene induction, cell cycle progression and cell signaling. We further show that these defects are caused by the inability of large cells to scale nucleic acid and protein biosynthesis in accordance with cell volume increase, which effectively leads to cytoplasm dilution. Finally, we determine why nucleic acid and protein biosynthesis do not scale with cell volume beyond a certain critical size. DNA becomes limiting. We conclude that the correct DNA to cytoplasm ratio is vital for many perhaps all cellular functions and that the range where this ratio supports optimal cell function is remarkably narrow.
2020-02-05 | GSE111075 | GEO
Project description:Cell size varies greatly between cell types, yet within a specific cell type and growth condition, cell size is narrowly distributed. Why maintenance of a cell-type specific cell size is important is not understood. Here we show that growing beyond a certain size has wide-ranging effects on cell physiology. Large cells are defective in gene induction, cell cycle progression and cell signaling. We further show that these defects are caused by the inability of large cells to scale nucleic acid and protein biosynthesis in accordance with cell volume increase, which effectively leads to cytoplasm dilution. Finally, we determine why nucleic acid and protein biosynthesis do not scale with cell volume beyond a certain critical size. DNA becomes limiting. We conclude that the correct DNA to cytoplasm ratio is vital for many perhaps all cellular functions and that the range where this ratio supports optimal cell function is remarkably narrow.
2019-02-07 | GSE110704 | GEO
Project description:This SuperSeries is composed of the SubSeries listed below.
2019-02-07 | GSE111076 | GEO
Project description:Proper organelle size is critical for many cell functions. However, how cells sense and control their organelle size remains elusive. Here, we develop a general model to study the size control of mitotic spindles by considering both extrinsic and intrinsic factors, such as the limited number of building blocks of the spindle, the interaction between the spindle and cell boundary, the DNA content, the forces generated by various molecular motors, and the dynamics of microtubules. We show that multiple pairs of chromatids, two centrosomes, and microtubules can self-assemble to form a mitotic spindle robustly. We also show that the boundary-sensing and volume-sensing mechanisms coexist in small cells, but both break down in large cells. Strikingly, we find that the upper limit of spindle length naturally arises from the geometric asymmetry of the spindle structure. Thus, our findings reveal, to our knowledge, a novel intrinsic mechanism that limits the organelle size.
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