Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Transcriptome analysis of Lippia rotundifolia using Roche 454 data.

PROVIDER: PRJNA436397 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6793272.fastq.gz	Fastqsanger.gz

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Transcriptome analysis of Lippia alba using Roche 454 data

| PRJNA436563 | ENA

Project description:Transcriptome of Lippia alba leaves using Roche 454 platform

| PRJNA555054 | ENA

Project description:Transcriptome of Lippia alba leaves using Roche 454 platform

| PRJNA534115 | ENA

Lippia alba cultivar:CL16

Project description:Transcriptome of Lippia alba leaves using Roche 454 platform

| PRJNA534110 | ENA

University of Edinburgh, Scotland

Project description:Teladorsagia circumcincta transcriptome sequencing study, using Roche 454-Titanium

| PRJEB2633 | ENA

High-throughput 16S rRNA amplicon pyrosequencing of contaminated aquifer sediment

Project description:Contaminated aquifer (Dusseldorf-Flinger, Germany) templates extracted from 5 sediment depths ranging between 6.4 and 8.4 m below ground and over 3 years of sampling were amplified for amplicon pyrosequencing using the primers Ba27f (5’-aga gtt tga tcm tgg ctc ag-3’) and Ba519r (5’- tat tac cgc ggc kgc tg-3’), extended as amplicon fusion primers with respective primer A or B adapters, key sequence and multiplex identifiers (MID) as recommended by 454/Roche. Amplicons were purified and pooled as specified by the manufacturer. Emulsion PCR (emPCR), purification of DNA-enriched beads and sequencing run were performed following protocols and using a 2nd generation pyrosequencer (454 GS FLX Titanium, Roche) as recommended by the developer. Quality filtering of the pyrosequencing reads was performed using the automatic amplicon pipeline of the GS Run Processor (Roche), with a slight modification concerning the valley filter (vfScanAllFlows false instead of TiOnly) to extract the sequences. Demultiplexed raw reads were furhter trimmed for quality and lenght (>250 bp).

2012-11-11 | GSE25345 | GEO

Project description:Transcriptome sequencing of Three Begonia species, using Roche 454 Titanium pyrosequencing.

| PRJEB2868 | ENA

Project description:Sparus aurata (Gilthead seabream) transcriptome sequencing study, using Roche 454-Titanium

| PRJEB2658 | ENA

RNA-seq transcriptomic profiling for self-renewing (SR) and senescent (SEN) human adult adipose derived mesenchymal stem cells (hADSCs)

Project description:Characterization of gene expression levels with RNA-seq was performed on self-renewing (SR) and senescent (SEN) human adult adipose derived mesenchymal stem cells (hADSCs) using the Roche 454 pyrosequencing platform.

2017-08-15 | GSE77284 | GEO

Limitations and possibilities of small RNA digital gene expression profiling: spleen and liver comparison (SOLiD)

Project description:Analysis of RNA samples by massive parallel sequencing holds the promise to assay gene expression in both a quantitative and qualitative fashion and therefore allows for digital gene expression (DGE) profiling. We assessed the effect of different experimental approaches by generating small RNA libraries from a biological sample as well as an equimolar pool of synthetic miRNAs and analyzed the results using capillary dideoxy sequencing and next-generation sequencing platforms (Roche/454, AB/SOLiD and Illumina/Solexa). Whereas different sequencing platforms provided highly similar results, large differences in DGE profiles were observed depending on the library preparation method used. Nevertheless, our results indicate that the preferential nature of the library preparation methods is systematic and highly reproducible and we show that DGE is well suited for the quantification of relative expression differences between samples. Keywords: Transcriptome analysis

2009-07-29 | GSE16371 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data