Project description:Transcriptome analysis of peritoneal macrophages infected with VSV (12h) and with or without pre-treatment of DiFMOC-G for 12 hours .
Project description:The peritoneal macrophages were infected with Mtb H37Rv for 4 hours, and the miRNA expression profile were analyzed with deep sequencing.
Project description:Infection of macrophages by Salmonella induces a transcriptional inflammatory response. The pattern of Salmonella induced genes is significantly altered by the expression of type I interferons. We used microArray analysis to identify changes in global gene expressioncaused by type I interferons following Salmonella infection
Project description:Exosomes derived from S. Typhimurium-infected RAW264.7 macrophages (24 and 48 hpi) were isolated by differential ultracentrifugation. Equal protein samples in triplicates were subjected to protein extraction and separation by SDS-PAGE. Protein bands excised from each lane were reduced, alkylated, and digested with trypsin. Peptide pools were desalted by C18 columns and analyzed by liquid chromatography-Orbitrap Fusion tandem mass spectrometry
Project description:The goal of this study is to perform transcriptome profiling of both infected and uninfected WT and HIF-1ɑ-/- peritoneal macrophages using RNA sequencing techniques. For that, WT and HIF-1ɑ-/- peritoneal macrophages were infeced for 6 hours with L. donovani promastigotes (or left uninfected as a control) and RNA samples were stored in TRI reagent (Sigma), for further analysis.
Project description:SrfJ is an effector of the type III secretion systems of the Gram-negative intracellular pathogen Salmonella enterica serovar Typhimurium. To study the effects of this effector on global gene expression in host cells, we have infected murine RAW264.7 macrophages with two strains of Salmonella enterica serovar Typhimurium. The comparison between cells infected with the wild-type strain and cells infected with a srfJ mutant revealed a number of genes that are differentially expressed when SrfJ is present.
Project description:To determine the underlying mechanisms by which trametinib affected LPS-induced inflammatory response in macrophages, we performed microarrays to define the global gene expression in murine peritoneal macrophages treating with trametinib or vehicle followed by LPS stimulation.
Project description:Salmonella enterica is one of the most important foodborne pathogens that infect a variety of animals and birds. In humans, S. Typhimurium causes gastroenteritis, leading to vomiting, diarrhea, fever, and abdominal cramps. We mainly get infected with Salmonella by ingesting comminated poultry products. Therefore, developing an oral live attenuated vaccine for the poultry industry is our best bet against Salmonella infection. In this article, we investigated the potential of the next generation of Salmonella vaccines. We generated a library of potentially attenuated S. Typhimurium mutants and compared fitness to that of a commercial vaccine. We also investigated the invasion and survival potential of these mutants in chicken macrophages. Our data indicate that although these mutants had no significant growth defects, they were much sensitive to macrophage attack. Analyzing the transcriptome data from infected primary chicken macrophages, we concluded that these mutants elicit a robust immune response by activating several immunoregulatory pathways. Our data also indicates that by combining phoPQ deletion with an already existing cya-crp deletion in MeganVac1, a much stronger immune response can be generated.