Project description:We used microarrays to detail the global programme of gene expression underlying the coculturing of degenerate NPCs and ASCs and identified distinct classes of altered genes and ncRNAs during this process.
Project description:We used microarrays to detail the global programme of gene expression underlying the coculturing of ASCs and degenerative NPCs and identified distinct classes of altered genes and ncRNAs during this process.
Project description:Chromatin immunoprecipitation followed by sequencing (ChIP-seq) was performed to investigate the epigenetic landscape in neural progenitor cells (NPCs) derived from L2HGA patient iPSCs with or without correction of the pathogenic L2HGDH variant. We profiled histone modifications H3K4me2 and H3K4me3 as well as input control across four conditions: unedited Patient 1 NPCs, corrected Patient 1 NPCs, corrected NPCs treated with DMSO, and corrected NPCs treated with the KDM5 inhibitor C70. This dataset provides insight into enhancer and promoter activity at the MYC locus and genome-wide effects of L-2HG-associated epigenetic dysregulation.
Project description:We analyzed the genome-wide binding of Sox2 and POU factor partner factors, Oct4 in ESCs (using published datasets PMID:18692474 and GSM307137, GSM307154, GSM307155) and Brn2 in NPCs. We found that Sox2 and Oct4 co-occupied a large subset of promoters and enhancers in ESCs, but that Sox2 and Brn2 co-occupy predominantly enhancers. Further, we overexpressed Brn2 in differentiating ESCs and showed that ectopic Brn2 recruited Sox2 to NPC-specific targets, resulting in skewed differentiation towards the neural lineage. Examination of transcription factor binding in ESCs, NPCs, and differentiating ESCs by ChIP-Seq.
Project description:Human adipose tissue contains two populations of progenitors (EPCs and ASCs) with cooperative roles in breast cancer. EPCs (CD45-CD34+CD31+CD13-CCRL2+) can generate endothelial cells. ASCs (CD45-CD34+CD31-CD13+CD140b+) are mesenchymal progenitors which generated pericytes. CD13+ cells and CD13- cells from 7 Lipotransfer aspirate
Project description:We indirectly cocultured OVCAR5-RFP cells using a 0.45 micron Boyden chamber with or without adipocytes for 4 days, collected the RNA from adipocytes, and performed RNA-seq
Project description:Human adipose tissue contains two populations of progenitors (EPCs and ASCs) with cooperative roles in breast cancer. EPCs (CD45-CD34+CD31+CD13-CCRL2+) can generate endothelial cells. ASCs (CD45-CD34+CD31-CD13+CD140b+) are mesenchymal progenitors which generated pericytes.