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TCR repertoire of effector CCR7-CD38+ cells accumulating during experimental infection with Typhoidal Salmonella

ABSTRACT: TCR repertoire of effector CCR7-CD38+ cells accumulating during experimental infection with Typhoidal Salmonella

PROVIDER: PRJNA450033 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6998310_1.fastq.gz	Fastqsanger.gz
	SRR6998310_2.fastq.gz	Fastqsanger.gz
	SRR6998311_1.fastq.gz	Fastqsanger.gz
	SRR6998311_2.fastq.gz	Fastqsanger.gz
	SRR6998312_1.fastq.gz	Fastqsanger.gz

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TCR repertoire of effector CCR7-CD38+ cells accumulating during experimental infection with Typhoidal Salmonella

Project description:In order to characterize pathogen specific T cell responses against Salmonella volunteers challenged with Salmonella enterica serovar Typhi (S. Typhi) or Salmonella Paratyphi A (S. Paratyphi). we used mass cytometry, to identify effector CD4+ T cells circulating during infection. We identified a population of CCR7-CD38+ cells accumulating during infection, and via unbiased single cell cloning and expansion we demonstrated that these CCR7-CD38+ cells are enriched in Salmonella specific T cells. In this experiment we performed TCR repertoire analysis of CCR7-CD38+ and CCR7-CD38- cells to determine the clonality of CCR7-CD38+ cells, the overlap between the repertoire of CCR7-CD38+ cells and of non-activated effector CCR7-CD38- cells, and to identify within CCR7-CD38+ and CCR7-CD38- cells the presence of the CDR3b TCR sequence of the pathogen specific T cell clones isolated from CCR7-CD38+ cells

2018-05-13 | GSE113112 | GEO

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