Dataset Information


TCR repertoire of effector CCR7-CD38+ cells accumulating during experimental infection with Typhoidal Salmonella

ABSTRACT: In order to characterize pathogen specific T cell responses against Salmonella volunteers challenged with Salmonella enterica serovar Typhi (S. Typhi) or Salmonella Paratyphi A (S. Paratyphi). we used mass cytometry, to identify effector CD4+ T cells circulating during infection. We identified a population of CCR7-CD38+ cells accumulating during infection, and via unbiased single cell cloning and expansion we demonstrated that these CCR7-CD38+ cells are enriched in Salmonella specific T cells. In this experiment we performed TCR repertoire analysis of CCR7-CD38+ and CCR7-CD38- cells to determine the clonality of CCR7-CD38+ cells, the overlap between the repertoire of CCR7-CD38+ cells and of non-activated effector CCR7-CD38- cells, and to identify within CCR7-CD38+ and CCR7-CD38- cells the presence of the CDR3b TCR sequence of the pathogen specific T cell clones isolated from CCR7-CD38+ cells Overall design: Analysis of the TCR repertoire of CCR7-CD38+ and CCR7-CD38+ CD4 T cells isolated from frozen PBMC after diagnosis of enteric fever in one volunteer challenged with Salmonella Typhi and one challenged with Salmonella Paratyphi A

INSTRUMENT(S): Illumina MiSeq (Homo sapiens)

SUBMITTER: Margarida Rei  

PROVIDER: GSE113112 | GEO | 2018-05-13


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Clonal analysis of Salmonella-specific effector T cells reveals serovar-specific and cross-reactive T cell responses.

Napolitani Giorgio G   Kurupati Prathiba P   Teng Karen Wei Weng KWW   Gibani Malick M MM   Rei Margarida M   Aulicino Anna A   Preciado-Llanes Lorena L   Wong Michael Thomas MT   Becht Etienne E   Howson Lauren L   de Haas Paola P   Salio Mariolina M   Blohmke Christoph J CJ   Olsen Lars Rønn LR   Pinto David Miguel Susano DMS   Scifo Laura L   Jones Claire C   Dobinson Hazel H   Campbell Danielle D   Juel Helene B HB   Thomaides-Brears Helena H   Pickard Derek D   Bumann Dirk D   Baker Stephen S   Dougan Gordon G   Simmons Alison A   Gordon Melita A MA   Newell Evan William EW   Pollard Andrew J AJ   Cerundolo Vincenzo V  

Nature immunology 20180620 7

To tackle the complexity of cross-reactive and pathogen-specific T cell responses against related Salmonella serovars, we used mass cytometry, unbiased single-cell cloning, live fluorescence barcoding, and T cell-receptor sequencing to reconstruct the Salmonella-specific repertoire of circulating effector CD4+ T cells, isolated from volunteers challenged with Salmonella enterica serovar Typhi (S. Typhi) or Salmonella Paratyphi A (S. Paratyphi). We describe the expansion of cross-reactive respons  ...[more]

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